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Science
Volumn 247, Issue 4942, 1990, Pages 568-571
Tumorigenicity in human melanoma cell lines controlled by introduction of human chromosome 6
Author keywords

[No Author keywords available]
Indexed keywords

MUS MUSCULUS; ANIMALIA;
EID: 0025176514     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.2300817     Document Type: Article
Times cited : (312)
References (49)
  • 43
    • 0022458323 scopus 로고
    • Cells grown on cover slips were fixed 5 min in cold methanol (4°C) and permeabilized by dipping in acetone. The primary antibody, HMB-45 was incubated for 30 min. Slides were then washed three times with phosphate-buffered saline (PBS). Secondary fluoresceinated rabbit antibody to mouse antigen was incubated for 30 min. Again the slides were washed three times with PBS and observed under a cover slip with an epifluorescent microscope. As described by Gown et al., HMB-45 recognizes 97% of malignant melanomas (both pigmented and nonpigmented) while failing to react with normal skin melanocytes. The antibody recognizes an unknown cytoplasmic antigen. In direct screening of tumor tissues, the HMB-45 antibody in general strains "epitheloid" melanoma cells more uniformly positive than more "sarcomatous" melanoma cells (analogous to the situation following the introduction of chromosome 6 into our cell lines)
    • (1986) Am. J. Pathol , vol.123 , pp. 195
    • Gown, A.M.1
  • 44
    • 0023621717 scopus 로고
    • M. Gottesman, Ed. Exponentially growing cells were harvested for karyotypic analysis as described previously [(Academic Press, New York,]. A minimum of 50 cells per line were analyzed, with results expressed according to International System for Human Cytogenetic Nomenclature (ISCN) recommendations
    • (1987) Methods in Enzymology , pp. 267-278
    • Trent, J.1    Thompson, F.2
  • 45
    • 84966193378 scopus 로고
    • The model chromosome number of the UACC-903 cell line was 47, including clonal structural alterations of chromosome 1 [t(l;ll)(q23;q23) and derinv(l) (p36q25)]; chromosome 11 [t(l;ll) and del(ll) (q23)]; and chromosome 17 [t(17;?)(q25;?)]. No evidence of chromosome 6 alterations were observed in any cell (Fig. 3A). The UACC-091 cell line had a hypertriploid modal chromosome number (78 chromosomes per cell) with clonal structural alterations of chromosome 1 [del(l)(p22)]; chromosome 3 [del(3)(ql3)]; chromosome 5 [t(5;?)(pl4;?)]; chromosome 6 [del(6)(ql6)]; chromosome 7 [iso(7q)]; chromosome 12 [del(12)(pl2)]; chromosome 13 [t(13:?)(pll;?)]; chromosome 15 [t(15;?)(pll;?)]; and chromosome 16 [t(16;?)(q25;?)]. Fig. 3A documents chromosome 1 and 6 alterations from UACC-091 and chromosome 1 alterations from the UACC-903 cell line.
    • (1985) Cytogenet. Cell Genet. , vol.21 , pp. 1

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.