Coupled reverse transcription-polymerase chain reaction (RT-PCR) as a sensitive and rapid method for isozyme genotyping

Gene

Volumn 93, Issue 2, 1990, Pages 271-275

  Mocharla, Hanna ^a   Mocharia, Raman ^a   Hodes, M E ^a  

^a INDIANA UNIVERSITY   (United States)

Author keywords

nucleotide sequence homology; oligodeoxyribonucleotide primers; Recombinant DNA; amylase genes

Indexed keywords

AMYLASE; ISOENZYME; RNA DIRECTED DNA POLYMERASE;

ARTICLE; GENE STRUCTURE; GENETIC ENGINEERING; GENETIC TRANSCRIPTION; HEREDITY; HUMAN; HUMAN CELL; METHODOLOGY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

AMYLASES; EXONS; HUMAN; ISOENZYMES; POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA-DIRECTED DNA POLYMERASE; SALIVARY GLANDS; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY, NUCLEIC ACID; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TRANSCRIPTION, GENETIC;

EID: 0025147221     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(90)90235-J     Document Type: Article

References (13)

1. Methods compared for determining total amylase activity and amylase isoenzymes in serum
2. Detection of HIV-1 RNA sequences by in vitro DNA amplification
3. A monoclonal antibody that specifically inhibits human salivary α-amylase
4. Primary structures of human pancreatic α-amylase gene: its comparison with human salivary α-amylase gene
5. Molecular Cloning
6. A novel senstiive fluorometric staining technique for the detection of DNA in RNA preparation
7. Production of salivary type α-amylase in human lung cancer
8. A novel from of tissue-specific RNA processing produces apolipoprotein-B48 in intestine
9. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
10. Expression of the human amylase genes: recent origin of a salivary amylase promoter from an actin pseudogene
11. Three methods compared for isoamylase separation in tissue homogenates
12. Survey of α-amylase activity and isoamylases in autopsy tissue