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Volumn 247, Issue 4943, 1990, Pages 715-718

Antigen presentation requires transport of MHC class I molecules from the endoplasmic reticulum

Author keywords

[No Author keywords available]

Indexed keywords

MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1;

EID: 0025020492     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.2137259     Document Type: Article
Times cited : (140)

References (32)
  • 13
    • 85027810094 scopus 로고
    • For construction of the E19-Vac recombinant, a 5424-bp DNA fragment encoding the Ad5 E3 region (provided by R. Ricciardi, Wistar Institute, Philadelphia, PA) was digested with Sac I and gel-purified to yield a 2214-bp fragment containing the E19 gene. The fragment was Ugated into the Sac I polylinker site of pTZ.18R, which was digested with Hinf I and gel-purified to yield a 733-bp fragment. The Hinf I fragment was blunt-ended with the Klenow fragment of DNA polymerase I and treated with calf intestinal phosphatase before being ligated into the Sma I site of pSCll. The resulting plasmid, pJCl, contains 109 bp of adenovirus DNA upstream from the E19 initiation codon and 145 bp of adenovirus DNA downstream from the E19 termination codon. CVI cells were co-transfected with wild-type Vac and the pJCl plasmid and subjected to selection for thymidine kinase-deficient virus. All infectious recombinant vaccinia virus stocks were grown from an isolated virus plaque in BSC cells and stored at -70°C(16).
    • (1985) Mol. Cell. Biol. , vol.101 , pp. 725
    • Chakrabarti, S.1    Brechling, K.2    Moss, B.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.