Purification and characterization of a non-reconstitutable azurin, obtained by heterologous expression of the Pseudomonas aeruginosa azu gene in Escherichia coli

BBA - Bioenergetics

Volumn 1019, Issue 3, 1990, Pages 283-292

  van de Kamp, Mart ^a   Hali, Frits C ^a   Rosato, Nicola ^b   Agro, Alessandro Finazzi ^b   Canters, Gerard W ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

^b UNIVERSITY OF ROME TOR VERGATA   (Italy)

Author keywords

(P. aeruginosa); Blue copper protein; Fluorescence; Gel electrophoresis; Heterologous expression; NMR, 1H

Indexed keywords

AZURIN;

ARTICLE; ESCHERICHIA COLI; FLUORESCENCE; GEL ELECTROPHORESIS; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN PURIFICATION; PSEUDOMONAS;

AZURIN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; ISOELECTRIC FOCUSING; MAGNETIC RESONANCE SPECTROSCOPY; PSEUDOMONAS AERUGINOSA; RECOMBINATION, GENETIC; SPECTROMETRY, FLUORESCENCE; SUPPORT, NON-U.S. GOV'T;

ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0024990129     PISSN: 00052728     EISSN: None     Source Type: Journal    
DOI: 10.1016/0005-2728(90)90206-J     Document Type: Article

References (39)

1. Structural Features of Azurin at 2.7 Å Resolution
2. Luminescence Quenching in Azurin
3. The Measurement and Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry
4. Time-resolved fluorescence of apoferritin and its subunits.