An immunocytochemical technique offering increased sensitivity and lowered cost with a streptavidin-horseradish peroxidase conjugate

Journal of Histotechnology

Volumn 12, Issue 4, 1989, Pages 273-277

  Cartun, Richard W ^a   Pedersen, Carl A ^a  

^a HARTFORD HOSPITAL   (United States)

Author keywords

Biotin; Immunoperoxidase; Sensitivity; Streptavidinhorseradish peroxidase

Indexed keywords

AVIDIN; BIOTIN; HORSERADISH PEROXIDASE;

ARTICLE; CYTOCHEMISTRY; IMMUNOPEROXIDASE STAINING; METHODOLOGY;

ARMORACIA RUSTICANA;

EID: 0024811825     PISSN: 01478885     EISSN: 20460236     Source Type: Journal    
DOI: 10.1179/his.1989.12.4.273     Document Type: Article

References (4)

1. Stemberger LA. Hardy PH Jr. Cauculis JJ, et al The unlabeled anti body-enzyme method of immunohis-tochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-anti-horseradish peroxidase) and its use in identification of spirochetes. J Histochem Cy toe hem 18:315, 1970.
2. Guesdon J-L. Temynack T. Avrameas S: The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem 27:1131, 1979.
3. Hsu SM. Raine L. Fänger H: Use of avidin-biotin-peroxidase complex (ABC) in im mu no peroxidase techniques. A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577. 1981.
4. Huang WM, Gibson SJ. Facer JG, et al. Improved section adhesion for immunocytochemistry using high molecular weight potvmers of L-lysine as a slide coating. Histochemistry' 77:275-279, 1983.