Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction

Genomics

Volumn 5, Issue 4, 1989, Pages 874-879

  Orita, Masato ^a   Suzuki, Youichi ^a   Sekiya, Takao ^a   Hayashi, Kenshi ^a  

^a NATIONAL CANCER CENTER RESEARCH INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; CELL LINE; GENE AMPLIFICATION; GENETIC POLYMORPHISM; GENETICS; MUTATION; NUCLEOTIDE REPEAT; NUCLEOTIDE SEQUENCE; ONCOGENE RAS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION;

BASE SEQUENCE; CELL LINE; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE AMPLIFICATION; GENES, RAS; MUTATION; POLYMERASE CHAIN REACTION; POLYMORPHISM (GENETICS); REPETITIVE SEQUENCES, NUCLEIC ACID; SUPPORT, NON-U.S. GOV'T;

EID: 0024756969     PISSN: 08887543     EISSN: 10898646     Source Type: Journal    
DOI: 10.1016/0888-7543(89)90129-8     Document Type: Article

References (21)

1. A general method for isolation of high molecular weight DNA from eukaryotes
2. Three different mutations in codon 61 of the human N-ras gene detected by synthetic oligonucleotide hybridization
3. Construction of a genetic linkage map in man using restriction fragment length polymorphisms
4. Source and evolution of human Alu repeated sequences
5. Mechanism of activation of an N-ras gene in the human fibrosarcoma cell line HT1080
6. Activation of Ki-ras 2 gene in human colon and lung carcinomas by two different point mutations
7. Isolation and regional localization of DNA segments revealing polymorphic loci from human chromosome 13
8. 9 globin allele by hybridization with synthetic oligonucleotides
9. Base sequence studies of 300 nucleotide renatured repeated human DNA clones
10. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus
11. Use of labeled primers in polymerase chain reaction (LP-PCR) for a rapid detection of the product
12. A gene deletion ending within a complex array of repeated sequences 3′ to the human beta-globin gene cluster
13. Sequence of the cDNA for angiogenin, a human angiogenesis factor
14. A ligase-mediated gene detection technique
15. Detection of single base substitutions in total genomic DNA
16. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms
17. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
18. The use of thin acrylamide gels for DNA sequencing
19. Four human carcinoma cell lines with novel mutations in position 12 of c-K-ras oncogene
20. Complete sequence and structure of the gene for human adenosine deaminase
21. A method to detect and characterize point mutations in transcribed genes; amplification and overexpression of the mutant c-Ki-ras allele in human tumor cells