Characterization of the Human Colon Carcinoma Cell Line (Caco-2) as a Model System for Intestinal Epithelial Permeability

Gastroenterology

Volumn 96, Issue 2, 1989, Pages 736-749

  Hidalgo, Ismael J ^a,b   Raub, Thomas J ^a,b   Borchardt, Ronald T ^a,b  

^a UNIVERSITY OF KANSAS   (United States)

^b PFIZER INC   (United States)

Author keywords

horseradish peroxidase; HRP; TEER; transepithelial electrical resistance

Indexed keywords

RADIOISOTOPE;

BIOLOGICAL MODEL; CELL CULTURE; CELL DIFFERENTIATION; CELL LINE; CELL TRANSPORT; COLON CARCINOMA; HUMAN; INTESTINE MUCOSA PERMEABILITY; PRIORITY JOURNAL; ULTRASTRUCTURE;

ADENOCARCINOMA; BIOLOGICAL TRANSPORT; CELL LINE; CELL MEMBRANE PERMEABILITY; COLONIC NEOPLASMS; CULTURE MEDIA; ELECTRIC CONDUCTIVITY; GASTROINTESTINAL MOTILITY; HORSERADISH PEROXIDASE; HUMAN; IN VITRO; INTESTINAL ABSORPTION; MEMBRANES, ARTIFICIAL; SUPPORT, NON-U.S. GOV'T; TUMOR CELLS, CULTURED;

EID: 0024593744     PISSN: 00165085     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0016-5085(89)80072-1     Document Type: Article

References (55)

1. In vitro drug absorption models. I. Brush border membrane vesicles, isolated mucosal cells and everted intestinal rings: characterization and salicylate accumulation
2. Intestinal metabolism of glutamine and glutamate from the lumen as compared to glutamine from blood
3. Intestinal drug absorption and metabolism I: comparison of methods and models to study physiological factors of in vitro and in vivo intestinal absorption
4. Separation and use of enterocytes
5. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria
6. Behaviour of isolated rat intestinal cells maintained in suspension or monolayer cultures
7. Enterocytic differentiation of cultured human colon cancer cells by replacement of glucose by galactose in the medium
8. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture
9. Classification of human colorectal adenocarcinoma cell lines
10. Establishment and description of a new line
11. Improvements in epoxy resin embedding methods
12. The use of lead citrate at high pH as an opaque stain in electron microscopy
13. Purification of the human intestinal brush border membrane
14. Ultrastructural localization of several phosphatases with cerium
15. Improved localization of intracellular sites of phosphatases using cerium and cell permeabilization
16. Migration of neutrophils across monolayers of cultured microvascular endothelial cells: an in vitro model of leukocyte extravasation
17. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy
18. Imidazole increases the sensitivity of the cytochemical reaction for peroxidase with diaminobenzidine at a neutral pH
19. Cell surface polarity in epithelia
20. Enterocytic differentiation and glucose utilization in the human colon tumor cell line Caco-2: modulation by forskolin
21. The cytochemical application of new potent inhibitors of alkaline phosphatases
22. Endocytosis in absorptive cells of cultured human small-intestinal tissue: horseradish peroxidase, lactoperoxidase, and ferritin as markers
23. Turnover of brush-border glycoproteins in human intestinal absorptive cells: do lysosomes have a regulatory function
24. Epithelial properties of human colonic carcinoma cell line Caco-2: electrical parameters
25. Functional morphology of the mucosa of the small intestine
26. Cell surface asymmetry is a prerequisite for the function of transporting and secreting epithelia
27. Presence and cell growth-related variations of glycogen in human colorectal carcinoma cell lines in culture
28. A lateral membrane protein in intestinal epithelial cells (abstr)
29. Cytochemical localization of alkaline phosphatase and Na'-pump sites in adult rat colon
30. Epithelial differentiation in the fetal rat colon. I. Plasma membrane phosphatase activities
31. Isolation of basolateral membranes from columnar cells of the proximal colon of the guinea pig
32. Enzyme patterns and protein absorption in rat colon during development
33. Common characteristics for Na'-dependent sugar transport in Caco-2 cells and human fetal colon
34. Receptor-mediated transport of insulin across endothelial cells
35. Transcellular transport of polymeric immunoglobulins by secretory components: a model system for studying intracellular protein sorting
36. Intracellular transport of newly synthesized secretory and membrane proteins
37. Distribution of immunoglobulin G receptors in the small intestine of the young rat
38. Gastrointestinal absorption of epidermal factor in suckling rats
39. Uptake and transepithelial transport of nerve growth factor in suckling rat ileum
40. Horseradish peroxidase transport across adult rabbit jejunum in vitro
41. Macromolecules can pass through occluding junctions of rat ileal epithelium during cholinergic stimulation
42. Apical and basolateral endocytosis in Madin-Darby canine kidney (MDCK) cells grown on nitrocellulose filters
43. Membrane-bound and fluid-phase macromolecules enter separate prelysosomal compartments in absorptive cells of suckling rat ileum
44. Macromolecular transport in the fetal rat intestine
45. Ionic conductances of extracellular shunt pathway in rabbit ileum. Influence of shunt in transmural sodium transport and electrical potential differences
46. Effect of temperature on the occluding junctions of monolayers of epithelioid cells (MDCK)
47. Contribution of solvent drag through intercellular junctions to absorption of nutrients by the small intestine of the rat
48. Physiological regulation of transepithelial impedance in the intestinal mucosa of rats and hamsters
49. Structural basis for physiological regulation of paracellular pathways in intestinal epithelia
50. A novel cell line (Caco-2) for the study of intestinal lipoprotein synthesis
51. Polarized secretion of newly synthesized lipoproteins by the Caco-2 human intestinal cell line
52. Development of vasoactive intestinal peptide-responsive adenylate cyclase during enterocyte differentiation of Caco-2 cells in culture
53. 57Co]Cyanocobalamin uptake by human colon adenocarcinoma cell line (Caco-2) (abstr)
54. Crypt cell antigen expression in human colon tumor cell lines: analysis with a panel of monoclonal antibodies to Caco-2 luminal membrane components