A Novel, Functional Variant of Cytochrome C: Replacement of the Histidine Ligand with Arginine via Site-Directed Mutagenesis

Journal of the American Chemical Society

Volumn 111, Issue 2, 1989, Pages 766-767

  Sorrell, Thomas N ^a   Martin, Patrick K ^a   Bowden, Edmond F ^b  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME C;

CELL CULTURE; FUNGUS; GENETIC ENGINEERING; MUTATION; NONHUMAN; PRELIMINARY COMMUNICATION; PROTEIN STRUCTURE;

EID: 0024534401     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja00184a074     Document Type: Article

References (19)

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3. There are two forms of cytochrome c in Saccharomyces cerevisciae termed iso-1 and iso-2. The less abundant (5% of the total cytochrome c) is the iso-2 protein: Sherman, R.; Stewart, J. W.; Helms, C.; Downie, T. A. Proc. Natl. Acad. Sci. U.S.A. 1978, 75, 1437-1441.
4. 4 The CYC7-H2 gene is a mutation in which a Tyl element has been inserted 5' to the iso-2-cytochrome c coding region of CYC7. The Tyl insertion causes a 20-fold increase in CYC7 expression in a and a haploid cell types of S. cerevisiae: Errede, B.; Cardillo, T. S.; Sherman, F.; Dubois, E.; Deschamps, J. Wiame, J.-M. Cell 1980, 22, 427-436.
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