Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Gene

Volumn 77, Issue 1, 1989, Pages 51-59

  Ho, Steffan N ^a   Hunt, Henry D ^a   Horton, Robert M ^a   Pullen, Jeffrey K ^a   Pease, Larry R ^a  

^a MAYO CLINIC   (United States)

Author keywords

Genetic engineering; major histocompatibility complex mutants; oligodeoxyribonucleotide primers; recombinant DNA; sequencing; Taq polymerase

Indexed keywords

MUTANT PROTEIN;

GENETIC ENGINEERING; MAJOR HISTOCOMPATIBILITY COMPLEX; METHODOLOGY; MOLECULAR CLONING; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMAL; DNA, RECOMBINANT; DNA-DIRECTED DNA POLYMERASE; EXONS; GENE AMPLIFICATION; GENES, MHC CLASS I; GENES, SYNTHETIC; GENETIC ENGINEERING; MICE; MOLECULAR SEQUENCE DATA; MUTATION; OLIGODEOXYRIBONUCLEOTIDES; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TAQ POLYMERASE;

EID: 0024520745     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(89)90358-2     Document Type: Article

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