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30
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-
0013043290
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We removed 1 to 12 μl (5 μl in most cases) of hemolymph from each worker. JH analyses were performed in Marseille, France, according to, J. Hoffmann and M. Porchet, Eds. (Springer-Verlag, Berlin, Other results obtained with this technique agree with titers determined by gas chromatography/mass spectrometry (GC/MS)
-
(1984)
Biosynthesis, Metabolism and Mode of Action of Invertebrate Hormones
, pp. 355-362
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Strambi, C.1
Strambi, A.2
de Reggi, M.3
Delaage, M.4
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32
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-
0018214757
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-
Samples were coded, allowing blind analyses. After separation with high-performance liquid chromatography, the immunoreactive product was found to have the same retention time as JH III diol, demonstrating that JH III was the only JH homolog in bee hemolymph. This result agrees with (17)
-
(1978)
Z. Naturforsch.
, vol.33
, pp. 847
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-
Hagenguth, H.1
Rembold, H.2
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35
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0023667059
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-
The colony was established as in (18), except 200 randomly selected workers were individually marked with colored, numbered plastic tags. One hundred tagged bees were treated topically with 200 μg of (RS) -methoprene (isopropyl (2R, 4E) -11-methoxy-3, 7, 1 l-trimethyl-2, 4-dodecadienoate) dissolved in 5 μl of acetone, and 100 were treated with acetone alone. Although recent evidence, suggests that there are different receptor sites for JH homologs and analogs in the tissue of at least one insect species (Manduca sexta), methoprene has demonstrated JH-like activity in many species, at the molecular
-
(1987)
Science
, vol.237
, pp. 999
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Prestwich, G.D.1
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37
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0016414629
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The efficacy of methoprene as a JH analog in honey bees is well established (15, 16). We quantified the incidence of precocious foraging among the two groups of tagged bees during daily 1-hour observation periods at the colony entrance, when bees were 5 to 10 days old. A census of tagged bees was also taken early in the morning of day 11, before the onset of foraging
-
(1975)
Annu. Rev. Entomol.
, vol.20
, pp. 417
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-
Staal, G.B.1
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45
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-
0003732928
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-
Allozymes of malate dehydrogenase-1 (Mdh) that occur naturally in populations of honey bees (29) were used as subfamily markers, as in (9). Three unrelated virgin queens were instrumentally inseminated, (Dadant and Sons, Hamilton, IL, with the semen of three unrelated drones. Each drone carried a different Mdh allozyme, designated “slow” (S), “medium” (M), and “fast” (F)
-
(1977)
Instrumental Insemination of Honey Bee Queens
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-
Laidlaw, H.H.1
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46
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0017625329
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-
Three single-cohort colonies were established (18) from the progeny of each queen. Use of allozyme markers allowed blind behavioral observations. Individuals sampled for JH titer determinations were among those used for allozyme analysis (with polyacrylamide gel electrophoresis)
-
(1977)
Biochem. Genet.
, vol.15
, pp. 859
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Contel, E.P.B.1
Mestriner, M.A.2
Martins, E.3
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50
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85027734236
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-
We thank I. Muller for translating articles, O. R. Taylor for providing pheromone swarm lures, G. B. Staal for providing methoprene, for assistance, H. H. Hagedorn for discussion, and D. L. Denlinger, J. F. Downhower, R. Nowogrodzki, T. D. Seeley, W. R. Tschinkel, and M. J. West-Eberhard for reviewing the manuscript. Supported by postdoctoral fellowships from NSF (BSR-8800227) and The Ohio State University (G. E. R.), NSF grant BNS-8719283 (R. E. P.), and the CNRS (C. S. and A. S.)
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-
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Bart, J.R.1
Calderone, N.W.2
Delbecque, J.P.3
Fondrk, M.K.4
Gianelos, J.A.5
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