Morphological and elemental integrity of freeze‐fractured, freeze‐dried cultured cells during ion microscopic analysis

Journal of Microscopy

Volumn 154, Issue 1, 1989, Pages 39-57

  Ausserer, Walter A ^a   Chandra, Subhash ^a   Morrison, George H ^a  

^a Department of Molecular Biology and Genetics   (United States)

Author keywords

calcium; diffusible ions; elemental analysis; Hurler's Syndrome; ion beam etching; ion microscopy; magnesium; matrix effects; mitochondria; potassium; SIMS; sodium

Indexed keywords

METAL;

ELEMENTAL ANALYSIS; FREEZE FRACTURE; HURLER SYNDROME; ION MICROANALYSIS; METHODOLOGY; MITOCHONDRION;

ANIMAL; CELLS, CULTURED; ELEMENTS; FIBROBLASTS; FREEZE FRACTURING; HUMAN; MICE; MICROSCOPY; MICROSCOPY, ELECTRON, SCANNING; PRESERVATION, BIOLOGICAL; RATS; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0024379285     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.1989.tb00566.x     Document Type: Article

References (26)

1. The application of liquid metal ion sources to ion‐microprobe secondary ion mass spectroscopy
2. High resolution imaging with the stigmatic ion microscope
3. Practical limitations in depth profiling of low energy implants into amorphised and crystalline silicon
4. Characterization and removal of ion yield transients in the near surface region of secondary ion mass spectrometry depth profiles
5. Quantitative microlocalization of diffusible ions in normal and galactose cataractous rat lens by secondary ion mass spectrometry
6. Matrix effects in secondary ion mass spectrometric analysis of biological tissue
7. Evaluation of matrix effects in ion microscopic analysis of freeze‐fractured, freeze‐dried cultured cells
8. Imaging elemental distribution and ion transport in cultured cells with ion microscopy
9. Imaging intracellular elemental distribution and ion fluxes in cultured cells using ion microscopy: a freeze‐fracture methodology
10. Probing mitochondria in living cells with rhodamine 123
11. A summary of ion beam etching of biological material with special reference to the saddle field source
12. Preferential sputtering effects in the ion microscopic microanalysis of biological tissues
13. Cellular microanalysis: a comparison between electron microprobe and secondary ion emission microanalysis
14. Quick‐freeze, deep‐etch preparation of samples for 3‐D electron microscopy
15. Uncoated observation and etching of non‐conductive material by ion beam bombardment in scanning electron microscopy
16. Le Microanalyseur ionique de seconde generation Cameca, Modele 3f
17. Progress in high resolution scanning ion mass spectrometry imaging microanalysis
18. SIMIPS: secondary ion mass image processing system
19. Ultrastructural comparison of ion beam and radiofrequency plasma etching effects on biological tissue sections
20. Ion beam etching effects in biological microanalysis
21. Differential sputtering correction for ion microscopy with image depth profiling
22. Calcium content of mitochondria and endoplasmic reticulum in liver frozen rapidly in vivo
23. Calcium release and ionic changes in the sarcoplasmic reticulum of tetanized muscle: an electron probe study
24. Electron probe analysis of vascular smooth muscle
25. On‐line ion implantation for quantification in secondary ion mass spectrometry: determination of trace carbon in thin layers of silicon