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(a) Gilbert, W. Nature 1986, 319, 618. (b) Cech, T. R. Proc. Natl. Acad. Sci. U.S.A. 1986, 83, 4360. (c) Benner, S. A.; Ellington, A. D. Proc. Natl. Acad. Sci. U.S.A. In press.
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2-Benzoyl-5'-dimethoxytrityl-d-iso-C diisopropyl phosphoramidite was prepared from d-iso-C by the general procedure of Atkinson and Smith: Atkinson, T.; Smith, M. Oligonucleotide Synthesis: A Practical Approach; Gait, M. J., Ed.; IRL Press: Oxford
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2-Benzoyl-5'-dimethoxytrityl-d-iso-C diisopropyl phosphoramidite was prepared from d-iso-C by the general procedure of Atkinson and Smith: Atkinson, T.; Smith, M. Oligonucleotide Synthesis: A Practical Approach; Gait, M. J., Ed.; IRL Press: Oxford, 1985; pp 35.
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Incorporation of d-iso-C into the synthetic DNA molecules was verified by digestion of template samples
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When this method was used, a small amount (ca. 15%) of dU was found to be present in the templates, presumably arising from the hydrolysis of d-iso-C under the alkaline conditions used for deprotecting the synthetic oligonucleotides
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Incorporation of d-iso-C into the synthetic DNA molecules was verified by digestion of template samples (Eritja, R., et al. Nucl. Acids Res. 1986, 14, 8135). When this method was used, a small amount (ca. 15%) of dU was found to be present in the templates, presumably arising from the hydrolysis of d-iso-C under the alkaline conditions used for deprotecting the synthetic oligonucleotides.
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