Puwainaphycin C, a Cardioactive Cyclic Peptide from the Blue-Green Alga Anabaena BQ-16-1. Use of Two-Dimensional 13C-13C and 13C-15N Correlation Spectroscopy in Sequencing the Amino Acid Units

Journal of the American Chemical Society

Volumn 111, Issue 16, 1989, Pages 6128-6132

  Moore, Richard E ^a   Bornemann, Volker ^a   Niemczura, Walter P ^a   Gregson, John M ^a   Chen, Jian Lu ^a   Patterson, Gregory M L ^a   Helms, Gregory L ^a   Norton, Ted R ^b  

^a UNIVERSITY OF HAWAII   (United States)

^b University of Hawaii Hilo   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PUWAINAPHYCIN C; PUWAINAPHYCIN D; UNCLASSIFIED DRUG;

DRUG ISOLATION; DRUG STRUCTURE; INFRARED SPECTROPHOTOMETRY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; ULTRAVIOLET SPECTROPHOTOMETRY;

EID: 0024324764     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja00198a021     Document Type: Article

References (12)

1. Norton, T. R.; Moore, R. E.; Patterson, G. M. L., unpublished results.
2. Entzeroth, M.; Moore, R. E.; Niemczura, W. P.; Patterson, G. M. L.; Shoolery, J. N. J. Org. Chem. 1986, 51, 5307–5310.
3. 100) the positive inotropic effect (PIE) is 325-550 with only a slight increase in chronotropic activity to 128. The PIE is not inhibited by propranolol and phentolamine and only partially inhibited by verapamil.
4. The puwainaphycins were named after the site of the Punchbowl National Cemetery (called Puowaina in Hawaiian) and the Hawaiian word for heart (puuwai).
5. The alga was isolated and grown in mass culture by using the procedure described for Hapalosiphon fontinalis [Moore, R. E.; Cheuk, C.; Yang, X.-Q.G.; Patterson, G. M. L.; Bonjouklian, R.; Smitka, T. A.; Mynderse, J. S.; Foster, R. S.; Jones, N. D.; Swartzendruber, J. K.; Deeter, J. B. J. Org. Chem. 1987, 52, 1036–1043].
6. Wagner, G. J. Magn. Reson. 1983, 55, 151–156. (b) Bax, A.; Drobny, G. J. Magn. Reson. 1985, 61, 306–320.
7. 6 are 25.9, 37.4, 66.3, 30.9, and 10.7 ppm.
8. Helms, G. L.; Moore, R. E.; Niemczura, W. P.; Patterson, G. M. L.; Tomer, K. B.; Gross, M. L. J. Org. Chem. 1988, 53, 1298–1307.
9. Niemczura, W. P.; Helms, G. L.; Chesnick, A. S.; Moore, R. E.; Bornemann, V. J. Magn. Reson. 1989, 81, 635–640.
10. 15N correlation in sequencing, see: Kainosho, M.; Tsuji, T. Biochemistry 1982, 21, 6273–6279.
11. 2 as the sole carbon source.
12. Bax, A. Two-Dimensional Nuclear Magnetic Resonance in Liquids; Delft University Press: Delft, Holland, 1982,