A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback

Chromosoma

Volumn 98, Issue 2, 1989, Pages 81-85

  Tautz, Diethard ^a   Pfeifle, Christine ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

RNA;

ARTHROPOD; DROSOPHILA; EMBRYO; EMBRYO DEVELOPMENT; GENETIC ENGINEERING; HEREDITY; IN SITU HYBRIDIZATION; METHODOLOGY; MUTANT; NONHUMAN;

ANIMAL; DNA PROBES; DROSOPHILA; GENE EXPRESSION REGULATION; GENES; INSECT HORMONES; NUCLEIC ACID HYBRIDIZATION; PROMOTER REGIONS (GENETICS); RNA; RNA, MESSENGER; SUPPORT, NON-U.S. GOV'T; TRANSCRIPTION, GENETIC; TRANSLATION, GENETIC;

EID: 0024319520     PISSN: 00095915     EISSN: 14320886     Source Type: Journal    
DOI: 10.1007/BF00291041     Document Type: Article

References (25)

1. The location of Ultrabithorax transcripts in Drosophila tissue sections
2. The molecular basis for metameric pattern in the Drosophila embryo
3. Identification and expression of the gap segmentation gene hunchback in Drosophila melanogaster
4. A gradient of bicoid protein in Drosophila embryos
5. The bicoid protein is a positive regulator of hunchback transcription in the early Drosophila embryo
6. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity
7. Studies of nuclear and cytoplasmic behavior during the five mitotic cycles that precede gastrulation in Drosophila embryogenesis
8. Organization of anterior pattern in the Drosophila embryo by the maternal gene bicoid
9. An improved in situ hybridization method for the detection of cellular RNAs in Drosophila tissue sections and its application for localizing transcripts of the homoeotic Antennapedia gene complex
10. Posterior segmentation of the Drosophila embryo in the absence of a maternal posterior organizer gene
11. The molecular genetics of embryonic pattern formation in Drosophila
12. Cloning and transcriptional analysis of the segmentation gene fushi tarazu of Drosophila
13. Methods of marking cells
14. Hunchback, a gene required for segmentation of an anterior and posterior region of the embryo
15. A molecular gradient in early Drosophila embryos and its role in specifying the body pattern
16. Abdominal segmentation of the Drosophila embryo requires a hormone receptor like protein encoded by the gap gene knirps
17. Mutations affecting segment number and polarity in Drosophila
18. Determination of Anteroposterior polarity in Drosophila
19. Molecular genetics of Krüppel, a gene required for segmentation of the Drosophila embryo
20. Differential regulation of the two transcripts from the Drosophila gap segmentation gene hunchback
21. Regulation of the Drosophila segmentation gene hunchback by two maternal morphogenetic centres
22. An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels
23. Fingerprotein of novel structure encoded by hunchback, a second member of the gap class of Drosophila segmentation genes
24. A molecular analysis of fushi tarazu, a gene in Drosophila melanogaster that encodes a product affecting embryonic segment number and cell fate
25. Looking at embryos