New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

Gene

Volumn 74, Issue 2, 1988, Pages 527-534

  Gietz, R Daniel ^a   Akio, Sugino ^a  

^a NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

Author keywords

cloning sites; mitotic stability; new alleles; Recombinant DNA; Saccharomyces cerevisiae; unique cuts

Indexed keywords

ALLELE; ESCHERICHIA COLI; FUNGUS; GENETIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE BASE; PRIORITY JOURNAL; RESTRICTION SITE; SACCHAROMYCES CEREVISIAE; YEAST;

ALLELES; DNA, RECOMBINANT; ESCHERICHIA COLI; GENES, FUNGAL; GENETIC VECTORS; GENOTYPE; MUTATION; OLIGODEOXYRIBONUCLEOTIDES; PLASMIDS; RESTRICTION MAPPING; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

EID: 0024266139     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(88)90185-0     Document Type: Article

References (22)

1. Plasmids pEMBLY: new single-stranded vectors for the recovery and analysis of yeast DNA sequences
2. Transformation of yeast by a replicating hybrid plasmid
3. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments
4. Construction of high copy vectors using 2 μm circle sequences
5. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene
6. Mitotic and meiotic stability of linear plasmids in yeast
7. Studies on transformation of Escherichia coli with plasmids
8. Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss
9. Yeast E. coli shuttle vectors with multiple unique restriction sites
10. Transformation of yeast
11. Transformation of intact yeast cells treated with alkali cations
12. Rapid and efficient site specific mutagenesis without phenotypic selection
13. Structure and sequence of the centromere DNA of chromosome 4 in Saccharomyces cerevisiae
14. Genetic applications of yeast transformation with linear and gapped plasmids
15. Vectors systems for the expression, analysis and cloning of DNA sequences in S. cerevisiae
16. Identification of a Ty insertion within the coding sequence of the S. cerevisiaeURA3 gene
17. High frequency transformation of yeast: autonomous replication of hybrid DNA molecules
18. Improved M 13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors