A novel method for liberating viral nucleic acid for assay of water samples with cDNA probes

Journal of Virological Methods

Volumn 22, Issue 1, 1988, Pages 13-21

  Richardson, Kenneth J ^a   Margolin, Aaron B ^a   Gerba, Charles P ^a  

^a UNIVERSITY OF ARIZONA   (United States)

Author keywords

Dot blot hybridization; Heat treatment; Human placental RNasin; Phenol chloroform extraction; Poliovirus

Indexed keywords

DRINKING WATER;

DNA PROBE; DOT HYBRIDIZATION; METHODOLOGY; NONHUMAN; ORGANIZATION AND MANAGEMENT; POLIOMYELITIS VIRUS; PRIORITY JOURNAL; VIRUS GENOME;

DNA; DNA PROBES; ENTEROVIRUS; HEAT; RIBONUCLEASES; RNA, VIRAL; SUPPORT, U.S. GOV'T, NON-P.H.S.; WATER MICROBIOLOGY;

EID: 0024230469     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-0934(88)90083-3     Document Type: Article

References (12)

1. Nonradioactive detection of RNA using the BluGENE system
2. Recent statistics of waterborne disease outbreaks
3. Detection of enteroviruses by spot hybridization
4. Viral heat resistance and infectious ribonucleic acid
5. Use of a cDNA dot-blot hybridization technique for detection of enteroviruses in water
6. Inhibition of ribonuclease efficacy of sodium dodecyl sulfate diethyl pyrocarbonate proteinase K and heparin using a sensitive ribonuclease assay
7. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
8. Laboratory methods for the growth and detection of animal viruses
9. Methods for detecting enteric viruses in water and wastewater
10. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose