Genetic engineering of Schizosaccharomyces pombe: A system for gene disruption and replacement using the ura4 gene as a selectable marker

MGG Molecular & General Genetics

Volumn 215, Issue 1, 1988, Pages 81-86

  Grimm, Christian ^a   Kohli, Jürg ^a   Murray, Johanne ^b   Maundrell, Kinsey ^c  

^a UNIVERSITY OF BERN   (Switzerland)

^b UNIVERSITY OF SUSSEX   (United Kingdom)

^c Chair and Division of Hematology   (Italy)

Author keywords

Gene disruption; Gene replacement; Schizosaccharomyces pombe; ura4 deletion; ura4 sequence

Indexed keywords

OROTIDINE 5' PHOSPHATE DECARBOXYLASE; RECOMBINANT DNA;

FUNGUS; GENETIC ENGINEERING; HEREDITY; MARKER RESCUE; NONHUMAN; SCHIZOSACCHAROMYCES POMBE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CHROMOSOME DELETION; DNA, FUNGAL; GENES, FUNGAL; GENES, STRUCTURAL; GENETIC ENGINEERING; GENETIC MARKERS; MOLECULAR SEQUENCE DATA; OROTIDINE-5'-PHOSPHATE DECARBOXYLASE; SACCHAROMYCETALES; SCHIZOSACCHAROMYCES; SUPPORT, NON-U.S. GOV'T; TRANSCRIPTION, GENETIC;

EID: 0024215989     PISSN: 00268925     EISSN: 16174623     Source Type: Journal    
DOI: 10.1007/BF00331307     Document Type: Article

References (21)

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14. Transformation of intact cells treated with alkali cations
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