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5
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85023367107
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Abbreviations: PMNs, polymorphonuclear lymphocytes
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4; HBSS, Hanks' balanced salt solution; GBSS, Gey's balanced salt solution
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4; HBSS, Hanks' balanced salt solution; GBSS, Gey's balanced salt solution.
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6
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0001733810
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0017355397
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These antibodies are prepared by injecting rabbits with 50 μg of fluorescein isothiocyanate derivative of albumin or of keyhole limpet hemocyanin in Freund's complete adjuvant
-
IgG is obtained by precipitation with 50% saturated ammonium sulfate, pH 7.0, and then dialysis versus phosphate-buffered saline or, when further purification is desired, versus 10 mM sodium phosphate buffer prior to chromatography on a DEAE-cellulose column.
-
These antibodies are prepared by injecting rabbits with 50 μg of fluorescein isothiocyanate derivative of albumin or of keyhole limpet hemocyanin in Freund's complete adjuvant, followed by boosters (25 μg of protein) at 1 and 2 months and thereafter at two-month intervals. IgG is obtained by precipitation with 50% saturated ammonium sulfate, pH 7.0, and then dialysis versus phosphate-buffered saline or, when further purification is desired, versus 10 mM sodium phosphate buffer prior to chromatography on a DEAE-cellulose column.
-
followed by boosters (25 μg of protein) at 1 and 2 months and thereafter at two-month intervals.
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28
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0019860746
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