The nitroreductase enzyme in walker cells that activates 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB 1954) to 5-(aziridin-1-YL)-4-hydroxylamino-2-nitrobenzamide is a form of NAD(P)H dehydrogenase (quinone) (EC 1.6.99.2)

Biochemical Pharmacology

Volumn 37, Issue 24, 1988, Pages 4671-4677

  Knox, Richard J ^a   Boland, Marion P ^a   Friedlos, Frank ^a   Coles, Brian ^b   Southan, Christopher ^b   Roberts, John J ^a  

^a INSTITUTE OF CANCER RESEARCH   (United Kingdom)

^b ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

5 (1 AZIRIDINYL) 2,4 DINITROBENZAMIDE; BENZAMIDE DERIVATIVE; DICOUMAROL; DRUG METABOLITE; MENADIONE; NITROBENZENE DERIVATIVE; NITROREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DEHYDROGENASE; WARFARIN;

5 (1 AZIRIDINYL) 4 HYDROXYLAMINO 2 NITROBENZAMIDE; ANIMAL CELL; CELL CULTURE; ENZYME ACTIVITY; NONHUMAN; PRIORITY JOURNAL; SPECTROMETRY; WALKER CARCINOMA;

AMINO ACID SEQUENCE; ANIMAL; ANTINEOPLASTIC AGENTS; AZIRIDINES; AZIRINES; BIOTRANSFORMATION; CARCINOMA 256, WALKER; MOLECULAR SEQUENCE DATA; NAD(P)H DEHYDROGENASE (QUINONE); NITROREDUCTASES; OXIDOREDUCTASES; QUINONE REDUCTASES; SPECTROPHOTOMETRY, ULTRAVIOLET; SUPPORT, NON-U.S. GOV'T;

EID: 0024205344     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/0006-2952(88)90336-X     Document Type: Article

References (32)

1. 2,4-dinitro-5-ethy- leneiminobenzamide (CB 1954): a potent and selective inhibitor of growth of the Walker carcinoma 256
2. CB 1954 (2,4-dinitro-5-aziridinyl benzamide) becomes a DNA interstrand crosslinking agent in Walker tumour cells
3. A new cytotoxic, DNA interstrand crosslinking agent, 5-(aziridin-l-yl)-4-hydroxylamino-2-nitrobenzamide, is formed from 5-(aziridin-l-yl)-2,4-dinitrobenzamide (CB 1954) by a nitroreductase enzyme in Walker carcinoma cells
4. Studies on the reversal of the selective antitumour effect of the aziridinyl derivative CB 1954 by 4-amino-5-imidazolecarboxamide
5. Studies on the protection by imidazoles against the cytotoxicity of the antitumour alkylating agents melphalan and CB 1954
6. A spectrophotometric method for the estimation of the carcinostatic agent, 5-aziridino-2,4-dinitrobenzamide (CB 1954), in biological fluids
7. NAD(P)H:menadione oxidoreductase. Novel purification of enzyme, cDNA and complete amino acid sequence, and gene regulation
8. Hydrolysis of riboflavin nucleotides in plasma monitored by high-performance liquid chromatography
9. Rat liver NAD(P)H:quinone reductase nucleotide sequence analysis of a quinone reductase cDNA clone and prediction of the amino acid sequence of the corresponding protein
10. 2 terminal sequence of the protein by double-stranded sequencing of exons 1 and 2
11. The mechanism of microsomal and mitochondria nitroreductase. Electron spin resonance evidence for nitroaromatic free radical intermediates
12. Tumour-growth inhibitory nitrophenylaziridines and related compounds structure-activity relationships
13. Tumour-growth inhibitory nitrophenylaziridines and related compounds structure-activity relationships II
14. The metabolism of the antitumour agent 1-(1-aziridinyl)-2,4-dinitrobenzene (CB 1837)
15. Soluble diaphorase in animal tissues
16. DT diaphorase: a historical review
17. Studies on the reaction mechanism of DT diaphorase. Action of dead-end inhibitors and effects of phospholipids
18. Properties and reaction mechanism of DT diaphorase from rat liver
19. Purification and characterisation of two isofunctional forms of NAD(P)H: quinone reductase from mouse liver
20. Rat liver cytosolic azoreductase. Electron transport properties and the mechanism of dicoumarol inhibition of the purified enzyme
21. Autoregulation plus positive and negative elements controlling transcription of genes in the [Ah] battery
22. Intracellular hydrogen transport in Ehrlich ascites tumor cells
23. The activity of the DT diaphorase in experimental hepatomas
24. The use of DT diaphorase for the detection of foci of early neoplastic transformation in rat liver
25. Chairman's summary
26. The metabolism of 4-nitroquinoline-1-oxide, a carcinogen III. An enzyme catalysing the conversion of 4-nitroquinoline-1-oxide to 4-hydroxyaminoquinoline-1-oxide in rat liver and hepatomas
27. DT diaphorase and the cancer cell
28. Meeting Report Twenty-first Paterson symposium bioactivation of quinone antitumour agents
29. The sensitivity of chemotherapeutic agents of a rat tumour grown in immunosuppressed mice
30. Antibody-directed enzymes revive anti-cancer pro-drugs concept