Cloning and comparative sequence analysis of the gene coding for isopenicillin N synthase in Streptomyces

MGG Molecular & General Genetics

Volumn 214, Issue 3, 1988, Pages 562-569

  Shiffman, Dov ^a   Mevarech, Moshe ^a   Jensen, Susan E ^b   Cohen, Gerald ^a   Aharonowitz, Yair ^a  

^a TEL AVIV UNIVERSITY   (Israel)

^b UNIVERSITY OF ALBERTA   (Canada)

Author keywords

lactam antibiotics; Cloning; Isopenicillin N synthase; Sequence similarity; Streptomyces

Indexed keywords

ENZYME; FUNGAL PROTEIN; ISOPENICILLIN N SYNTHETASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; CODON; COMPARATIVE STUDY; DNA PROBE; ENZYMOLOGY; GENETICS; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; RESTRICTION MAPPING; SOUTHERN BLOTTING; SPECIES DIFFERENCE; STREPTOMYCES;

AMINO ACID SEQUENCE; BASE SEQUENCE; BLOTTING, SOUTHERN; CLONING, MOLECULAR; CODON; COMPARATIVE STUDY; DNA PROBES; ENZYMES; FUNGAL PROTEINS; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; RESTRICTION MAPPING; SPECIES SPECIFICITY; STREPTOMYCES; SUPPORT, NON-U.S. GOV'T;

EID: 0024116545     PISSN: 00268925     EISSN: 16174623     Source Type: Journal    
DOI: 10.1007/BF00330495     Document Type: Article

References (28)

1. Penicillin biosynthesis: active site mapping with aminoadipyl-cysteinyl-valine variants
2. δ-(L-α-aminoadipyl)- L-cysteinyl- D-valine synthetase (ACV synthetase): a multifunctional enzyme with broad substrate specificity for the synthesis of penicillin and cephalosporin precursors
3. The relationship between base composition and codon usage in bacterial genes and its use in the simple and reliable identification of protein coding sequences
4. A rapid alkaline extraction procedure for screening recombinant plasmid DNA
5. Cloning and expression of the isopenicillin N synthetase is gene from Penicillium chrysogenum
6. Analysis of gene control signals by DNA fusion and cloning in E. coli
7. Purification and characterization of the isopenicillin N synthetase of Streptomyces lactamdurans
8. Strain improvement and preservation of β-lactam producing microorganisms
9. The red plaque test: a rapid method for identification of excision defective variants of bacteriophage lambda
10. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing
11. Biosynthesis of cephalosporins
12. Purification of isopenicillin N synthetase from Streptomyces clavuligerus
13. Activation of phenoxazinone synthase expression in Streptomyces lividans by cloned DNA sequence from Streptomyces antibioticus
14. Cell-free conversion of δ-(L-α-aminoadipyl)- L-cysteinyl- D-valine into an antibiotic with the properties of isopenicillin N in Cephalosporium acremonium
15. Cloning and nucleotide sequence determination of the isopenicillin N synthetase gene from Streptomyces clavuligerus
16. Rapid and sensitive protein similarity searches
17. Homology between Streptomyces genes coding for synthesis of different polyketides used to clone antibiotic biosynthetic genes
18. New M13 vectors for cloning
19. A bacteriophage lambda vector for cloning with BamHI and Sau3A
20. Purification of isopenicillin N synthetase
21. Cloning and characterization of the isopenicillin N synthetase gene mediating the formation of the β-lactam ring in Aspergillus nidulans
22. Isopenicillin N synthetase of Penicillium chrysogenum an enzyme that converts δ-(L-α-aminoadipyl)- L-cysteinyl- D-valine to isopenicillin N
23. Isolation, sequence determination and expression in Escherichia coli of the isopenicillin N synthetase gene from Cephalosporium acremonium
24. Analysis of the role of cysteine residues in isopenicillin N synthetase activity by site-directed mutagenesis
25. Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13mp18 and pUC19 vectors