Enumeration of IFN-γ-producing human lymphocytes by spot-ELISA. A method to detect lymphokine-producing lymphocytes at the single-cell level

Journal of Immunological Methods

Volumn 111, Issue 1, 1988, Pages 25-29

  Versteegen, Jannemieke M T ^a   Logtenberg, Ton ^a   Ballieux, Rudy E ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

Interferon ; Lymphokine; Single cell assay; spot ELISA

Indexed keywords

GAMMA INTERFERON;

CELL CULTURE; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; METHODOLOGY; NORMAL HUMAN; PRIORITY JOURNAL; T LYMPHOCYTE;

ADULT; CELL-FREE SYSTEM; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMAN; INTERFERON TYPE II; LYMPHOCYTE ACTIVATION; T-LYMPHOCYTES;

EID: 0023940215     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(88)90055-5     Document Type: Article

References (11)

1. Phenotypic characterization of individual interferon-gamma-producing cells after OKT3 antibody activation
2. Functional properties of human B cell subpopulations defined by monoclonal antibodies HB4 and FMC7
3. Activated T cells in human peripheral blood: quantitation with a reverse hemolytic plaque assay
4. An assay for colony-stimulating factor (CSF) production by single T lymphocytes: estimation of the frequency of cells producing granulocyte-macrophage CSF and multi-lineage CSF within a T lymphocyte clone
5. A hemolytic plaque assay for activated murine T cells
6. Kinetics and distribution of antigen-specific IgE-secreting cells during the primary antibody response in the rat
7. The ELISA-plaque assay for the detection and enumeration of antibody-secreting cells. An overview
8. Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA
9. Estimation by limiting dilution analysis of human IL-2-secreting T cells: detection of IL-2 produced by single lymphokine-secreting T cells
10. Measurement of the humoral immune response against Streptococcus pneumoniae type 3 capsular polysaccharide and oligosaccharide containing antigens by ELISA and ELISPOT techniques