Total chemical synthesis of a gene for hepatitis B virus core protein and its functional characterization

Gene

Volumn 66, Issue 2, 1988, Pages 279-294

  Nassal, Michael ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

dcm methylation; Recombinant DNA; restriction fragment replacement; site directed mutagenesis; synthetic gene; terminator

Indexed keywords

RADIOISOTOPE; SYNTHETIC DNA; VIRUS PROTEIN;

ANIMAL CELL; CELL CULTURE; ESCHERICHIA COLI; GENE EXPRESSION; GENE STRUCTURE; GENETIC ENGINEERING; HEPATITIS B VIRUS; HEREDITY; MAMMAL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RESTRICTION FRAGMENT; VIRUS CORE;

BASE SEQUENCE; BLOTTING, WESTERN; CLONING, MOLECULAR; DNA LIGASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENES, SYNTHETIC; GENES, VIRAL; GENETIC VECTORS; HEPATITIS B VIRUS; MOLECULAR SEQUENCE DATA; MUTATION; PLASMIDS; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SUPPORT, NON-U.S. GOV'T; VIRAL CORE PROTEINS;

EID: 0023902532     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(88)90364-2     Document Type: Article

References (51)

1. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique EcoRI sites for selection of EcoRI-generated recombinant DNA molecules
2. Gene synthesis machines: DNA chemistry and its uses
3. Molecular biology of hepatitis B viruses
4. Production of hepatitis B virus in vitro by transient expression of cloned HBV DNA in a hepatoma cell line
5. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA
6. Translational frameshifting: where will it stop?
7. Total synthesis of a gene for bovine rhodopsin
8. The molecular biology of hepatitis B viruses
9. A new technique for the assay of infectivity of human adenovirus 5 DNA
10. Studies on transformation of Escherichia coli with plasmids
11. Techniques for transformation of E. coli
12. Expression and replication of the hepatitis B virus genome under foreign promoter control
13. DNA polymerase associated with human hepatitis B virus antigen
14. Total synthesis of a gene
15. Human hepato-cellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen
16. Expression of mature foot and mouth disease antigen in E. coli
17. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
18. Specific amino acid substitutions in bacterioopsin: replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons
19. New specificities in Australia antigen positive sera distinct from Le Bouvier determinants
20. Molecular Cloning
21. Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K-12
22. Sequencing end-labeled DNA with base-specific chemical cleavages
23. Expression of hepatitis B virus surface and core antigens: influences of pre-S and precore sequences
24. Proteolytic self-cleavage of hepatitis B virus core protein may generate serum e antigen
25. Cloning, Expression and Characterization of Hepatitis B Virus Nucleocapsid Proteins
26. Structure-function studies on bacteriorhodopsin, III. Total synthesis of a gene for bacterio-opsin and its expression in Escherichia coli
27. Electron microscopy of human hepatitis B virus cores by negative-staining — carbon film technique
28. Hepatitis B virus gene function: the precore region targets the core antigen to cellular membranes and causes the secretion of the e antigen
29. Hepatitis B virus genes and their expression in Escherichia coli
30. Transcription termination and the regulation of gene expression
31. Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication
32. Inducible high level synthesis of mature fibroblast interferon in E. coli.
33. Expression of hepatitis B viral core region in mammalian cells
34. Gene Products of Hepatitis B Virus: Structure, Assignment to the Genoine and Biosynthesis
35. The duck hepatitis B virus preC region encodes a signal sequence which is essential for the synthesis and secretion of processed core proteins but not for virus formation
36. Production of hepatitis B virus particles in HepG2 cells transfected with cloned hepatitis B virus DNA
37. The codon adaptation index — a measure of directional synonymous codon usage bias, and its potential applications
38. High-level expression of human interferon gamma in Escherichia coli under control of the promoter of bacteriophage lambda
39. Hepatitis B core antigen: synthesis in E. coli and application in diagnosis
40. Production of hepatitis B virus by a differentiated human hepatoma cell line after transfection with cloned circular HBV DNA
41. Immuno-chemical structure of hepatitis B e antigen in the serum
42. Syringe method for the stepwise chemical synthesis of oligonucleotides
43. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications
44. Stable expression and replication of hepatitis B virus genome in an integrated state in a human hepatoma cell line transfected with the cloned viral DNA
45. Precore sequence of hepatitis B virus inducing e antigen and membrane association of the viral core protein
46. Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites
47. Mismatch and blunt to protruding end joining by DNA ligases
48. Infectious hepatitis B virus from cloned DNA of known nucleotide sequence
49. Putative reverse transcriptase intermediates of human hepatitis B virus in primary liver carcinomas
50. Hepatitis B virus particles are produced in a cell culture system by transient expression of transfected HBV DNA
51. Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors