Analysis of glucocorticoid actions on rat thymocyte deoxyribonucleic acid by fluorescence-activated flow cytometry

Endocrinology

Volumn 122, Issue 5, 1988, Pages 2158-2164

  Compton, Mark M ^a,b   Haskill, J Stephen ^a   Cidlowski, John A ^a  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEXAMETHASONE; GLUCOCORTICOID; MIFEPRISTONE;

ANIMAL CELL; ANIMAL EXPERIMENT; CELL CULTURE; DNA SYNTHESIS; FLOW CYTOMETRY; METHODOLOGY; NONHUMAN; RAT; THYMOCYTE; TISSUE CULTURE;

ACRIDINE ORANGE; ADRENALECTOMY; ANIMAL; CELL SURVIVAL; CELLS, CULTURED; CYCLOHEXIMIDE; DACTINOMYCIN; DEXAMETHASONE; DNA; DNA REPLICATION; ESTRENES; FLOW CYTOMETRY; KINETICS; MALE; MIFEPRISTONE; RATS; SUPPORT, U.S. GOV'T, P.H.S.; THYMUS GLAND;

EID: 0023894849     PISSN: 00137227     EISSN: 19457170     Source Type: Journal    
DOI: 10.1210/endo-122-5-2158     Document Type: Article

References (27)

1. Munck A, Guyre PM, Holbrook N 1984 Physiological functions of glucocorticoids in stress and their relation to pharmacological actions. Endocr Rev 5:25
2. Munck A, Crabtree GR 1981 Glucocorticoid-induced lymphocyte death. In: Bowen ID, Lockshin RA (eds) Cell Death in Biology and Pathology. Chapman and Hall, New York, pp 329-359
3. MacDonald RG, Cidlowski JA 1982 Glucocorticoids inhibit precursor incorporation into protein in splenic lymphocytes by stimulating protein degradation and expanding extracellular amino acid pools. Biochim Biophys Acta 717:236
4. MacDonald RG, Martin TP, Cidlowski JA 1980 Glucocorticoids stimulate protein degradation in lymphocytes: A possible mechanism of steroid-induced cell death. Endocrinology 107:1512
5. Cidlowski JA 1982 Glucocorticoids stimulate ribonucleic acid degradation in isolated rat thymic lymphocytes in vitro.Endocrinology111:184
6. Compton MM, Cidlowski JA 1986 Rapid in vivo effects of glucocorticoids on the integrity of rat lymphocyte genomic deoxyribonucleic acid. Endocrinology 118:38
7. Cohen JJ, Duke D. 1984 Glucocorticoid activation of a calcium dependent endonuclease in thymocyte nuclei leads to cell death. J Immunol 132:38
8. Vedeckis WV, Bradshaw HD 1983 DNA fragmentation in S49 lymphoma cells killed with glucocorticoids and other agents. Mol Cell Endocrinol 30:215
9. Vanderbilt JN, Bloom KS, Anderson JN 1982 Endogenous nuclease: properties and effects on transcribed genes in chromatin. J Biol Chem 257:13018
10. Umansky SR, Korol BA, Nelipovich PA 1981 In vivo DNA degradation in thymocytes of 7-irradiated or hydrocortisone-treated rats. Biochim Biophys Acta 655:9
11. Wyllie, AH 1980 Glucocorticoid-induced thymocyte apoptosis is associated with endonuclease activation. Nature 284:555
12. Robbins E, Marcus PI 1963 Dynamics of acridine orange-cell interaction. I. Interrelationships of acridine orange particles and cytoplasmic reddening. J Cell Biol 18:237
13. Moguilewsky M, Philibert D 1984 RU 38486: potent antiglucocor-ticoid activity correlated with strong binding to the cytosolic glucocorticoid receptor followed by impaired activation. J Steroid Biochem 20:271
14. Duval D, Durant S, Homo-Delarche F 1984 Effect of antiglucocorticoids on dexamethasone-induced inhibition of uridine incorporation and cell lysis in isolated mouse thymocytes. J Steroid Biochem 20:283
15. Thomas N, Edwards JL, Bell PA 1983 Studies of the mechanism of glucocorticoid-induced pyknosis in isolated rat thymocytes. J Steroid Biochem 18:519
16. Purnell MR, Whish WJD 1980 Novel inhibitors of poly (ADP-ribose) synthetase. Biochem J 185:775
17. Hayaishi O, Ueda K 1977 Poly (ADP-ribose) and ADP-ribosylation of proteins. Annu Rev Biochem 46:95
18. 3 cells. Arch Biochem Biophys 201:174
19. Miller OJ, Miller DA, Alderdice PW, Dev VG, Grewal MS 1971Quinacrine fluorescent karyotype of human diploid and heteroploid cell lines. Cytogenetics 10:338
20. Darzynkiewicz Z, Traganos F, Kapuscinski J, Stiano-Coico L, Melamed MR 1984 Accessibility of DNA in situ to various fluorochromes: relationship to chromatin changes during erythroid differentiation of Friend leukemia cells. Cytometry 5:355
21. Traganos F, Darzynkiewicz Z, Sharpless T, Melamed MR 1979Erythroid differentiation of Friend leukemia cells as studied by acridine orange staining and flow cytometry. J Histochem Cyto-chem 27:388
22. Kernell AM, Bolund L, Ringertz NR 1971 Chromatin changes during erythropoiesis. Exp Cell Res 65:1
23. Berger NA, Berger SJ, Sudar DC, Distelhorst CW 1987 The role of NAD and ATP in glucocorticoid induced cytotoxicity in susceptible lymphoid cells. J Clin Invest 79:1558
24. Borthwick NM, Bell PA 1982 Temporal relationships between inhibitory effects of glucocorticoids on cells of the CEM-C7 human leukaemic lymphoblast cell line. Eur J Clin Oncol 18:1093
25. Wielckens K, Delfs T 1986 Glucocorticoid-induced cell death and poly [adenosine diphosphate (ADP)-ribosyl]ation: increased toxicity of dexamethasone on mouse S49.1 lymphoma cells with the poly (ADP-Ribosyl)ation inhibitor benzamide. Endocrinology119:2383
26. Afanas’ev VN, Korol BA, Mantsygin YA, Nelipovich PA, Pechat-nikov VA, Umansky SR 1986 Flow cytometry and biochemical analysis of DNA degradation characteristic of two types of cell death FEBS Lett 194:347
27. Compton MM, Cidlowski JA 1987 Identification of a glucocorticoid-induced nuclease in thymocytes: A potential “lysis gene” product. J Biol Chem 262:8288