Comparisons of two in vitro cytotoxicity assays-The neutral red (NR) and tetrazolium MTT tests

Toxicology in Vitro

Volumn 2, Issue 1, 1988, Pages 1-6

  Borenfreund, E ^a   Babich, H ^a   Martin Alguacil, N ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2,4 DINITROPHENOL; 4 CHLOROMERCURIBENZOIC ACID; ANTIBIOTIC AGENT; ANTINEOPLASTIC AGENT; ASCORBIC ACID; AZACITIDINE; CAFFEINE; CHLOROQUINE; CITRININ; CYTARABINE; DACTINOMYCIN; DOXORUBICIN; FLUOROURACIL; FOLINATE CALCIUM; METHOTREXATE; MITOMYCIN C; NEUTRAL RED; OLIGOMYCIN; ORGANOTIN COMPOUND; PESTICIDE; PHENOBARBITAL; PHENYLHYDRAZONE; PROPRANOLOL; SODIUM AZIDE; TETRAZOLIUM; UNCLASSIFIED DRUG;

ANIMAL CELL; BIOASSAY; CYTOTOXICITY; FIBROBLAST; METHODOLOGY; MOUSE; NONHUMAN; TOXICITY TESTING;

EID: 0023874109     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/0887-2333(88)90030-6     Document Type: Article

References (26)

1. In vitro cytotoxicity of organic pollutants to bluegill sunfish (BF-2) cells
2. Structure—activity relationship (SAR) models established in vitro with the neutral red cytotoxicity assay
3. Polycyclic aromatic hydrocarbon in vitro cytotoxicity to bluegill BF-2 cells: mediation by S-9 microsomal fraction and temperature
4. In vitro cytotoxicity of polychlorinated biphenyls (PCBs) and toluenes to cultured bluegill sunfish BF-2 cells
5. In vitro cytotoxicity of metals to bluegill (BF-2) cells
6. “Rescue” techniques in cancer chemotherapy: use of leucovorin and other rescue agents after methotrexate treatment
7. In vitro cytotoxicity of heavy metals, acrylamide, and organotin salts to neural cells and fibroblasts
8. A simple quantitative procedure using monolayer culture for cytotoxicity assays (HTD/NR-90)
9. Toxicity determined in vitro by morphological alterations and neutral red absorption
10. Cytotoxicity of metals, metal-metal, and metal-chelator combinations assayed in vitro
11. Short-term quantitative in vitro cytotoxicity assays involving an S-9 activating system
12. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing
13. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of radiosensitivity
14. Rapid chemosensitivity testing of human lung tumor cells using the MTT assay
15. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability
16. Use of MTT colorimetric assay to measure cell activation
17. Rapid colorimetric assay for cell viability: application to the quantitation of cytotoxic and growth inhibitory lymphokines
18. Use of the 3T3 cell-neutral red uptake assay for irritants as an alternative to the rabbit (Draize) test
19. Pulmonary and generalized lysosomal storage induced by amphiphilic drugs
20. The ATP assay is more sensitive than the succinate dehydrogenase inhibition test for predicting cell viability
21. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays
22. An evaluation of three in vitro cytotoxicity assays
23. In vitro cytotoxicity assays: potential alternatives to the Draize test
24. Comparison of several alternative assays for measuring potential toxicants
25. Progress and problems in evaluating and validating alternative assays in toxicology
26. An improved colorimetric assay for interleukin 2