Nuclear protein migration involves two steps: Rapid binding at the nuclear envelope followed by slower translocation through nuclear pores

Cell

Volumn 52, Issue 5, 1988, Pages 655-664

  Richardson, W D ^a   Mills, A D ^b   Dilworth, S M ^b   Laskey, R A ^b   Dingwall, C ^b  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYGLUCOSE; SODIUM AZIDE;

ANIMAL CELL; CELL CULTURE; CONTROLLED STUDY; CYTOLOGY; MONKEY; NONHUMAN; OOCYTE; PRIORITY JOURNAL; TOAD; ULTRASTRUCTURE; VERO CELL; XENOPUS LAEVIS;

ADENOSINE TRIPHOSPHATE; ANIMAL; AZIDES; BIOLOGICAL TRANSPORT; CELL NUCLEUS; COLD; CYTOPLASM; DEOXYGLUCOSE; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GOLD; HISTOCYTOCHEMISTRY; MICROSCOPY, ELECTRON; NUCLEAR ENVELOPE; NUCLEAR PROTEINS; OOCYTES; PEPSIN A; PEPTIDE FRAGMENTS; SODIUM AZIDE; SUPPORT, NON-U.S. GOV'T; VERO CELLS; XENOPUS LAEVIS;

EID: 0023852444     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/0092-8674(88)90403-5     Document Type: Article

References (32)

1. Identification and characterization of a nuclear pore complex protein
2. Localization of gold in biological tissue
3. Two complexes that contain histones are required for nucleosome assembly in vitro role of nucleoplasmin and N1 in Xenopus egg extracts
4. Protein import into the cell nucleus
5. A polypeptide domain that specifies migration of nucleoplasmin into the nucleus
6. Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals
7. Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin
8. The nuclear annuli as pathways for nucleocytoplasmic exchanges
9. Binding within the nuclear annuli and its possible effect on nucleocytoplasmic exchanges
10. The effect of the electron-opaque pore material on exchanges through the nuclear annuli
11. The binding characteristics of the nuclear annuli
12. Movement of a karyophilic protein through the nuclear pores of oocytes
13. On the universality of nuclear pore complex structure
14. The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. 1. The mature oocyte
15. The nuclear envelope and the architecture of the nuclear periphery
16. Microinjection of early SV40 DNA fragments and T antigen
17. Fine structure of annulate lamellae
18. Co-existence of two different types of soluble histone complexes in nuclei of Xenopus laevis oocytes
19. A major soluble acidic protein located in nuclei of diverse vertebrate species
20. Assembly of SV40 chromatin in a cell-free system from Xenopus eggs
21. Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA
22. Transport and assembly of nuclear proteins
23. Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation
24. In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins
25. Adenovirus early gene regulation and the adeno-associated virus helper effect
26. A one-step purification of membrane proteins using a high efficiency immuno matrix
27. Intracellular transport of a karyophilic protein
28. Sizing of protein A-colloidal gold probes for immunoelectron microscopy
29. The fine structure of annulate lamellae
30. A large particle associated with the perimeter of the nuclear pore complex
31. Fine structural changes during sea urchin oogenesis
32. An electron microscopy study of the nuclear envelope of amphibian oocytes