Mutation of the insulin receptor at tyrosine 960 inhibits signal transmission but does not affect its tyrosine kinase activity

Cell

Volumn 54, Issue 5, 1988, Pages 641-649

  White, Morris F ^a   Livingston, James N ^a   Backer, Jonathan M ^a   Lauris, Vilma ^a   Dull, Thomas J ^b   Ullrich, Axel ^b   Kahn, C Ronald ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INSULIN RECEPTOR; PROTEIN TYROSINE KINASE;

ANIMAL CELL; CELL CULTURE; CONTROLLED STUDY; FEMALE; HAMSTER; MUTATION; NONHUMAN; OVARY CELL; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; ANIMAL; CELL LINE; CLONING, MOLECULAR; DNA; GENES, STRUCTURAL; HUMAN; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; PHOSPHORYLATION; PROTEIN-TYROSINE KINASE; RECEPTOR, INSULIN; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TRANSFECTION; TYROSINE;

EID: 0023814924     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(88)80008-4     Document Type: Article

References (51)

1. Insulin-activated tyrosine phosphorylation of a 15-kilodalton protein in intact 3T3-L1 adipocytes
2. Human insulin receptors mutated at the ATP-binding site lack protein tyrosine kinase activity and fail to mediate postreceptor effects of insulin
3. Analysis of insulin action using differentiated and dedifferentiated hepatoma cells
4. Insulin receptor regulation and desensitization in rat hepatoma cells
5. Expression of a functional human insulin receptor from a cloned cDNA in Chinese hamster ovary cells
6. The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling
7. Replacement of lysine residue 1030 in the putative ATP-binding region of the insulin receptor abolishes insulin- and antibody-stimulated glucose uptake and receptor kinase activity
8. Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose
9. Heterologous transmembrane signaling by a human insulin receptor—v-ros hybrid in Chinese hamster ovary cells
10. The cluster tray method for rapid measurement of solute fluxes in adherent cultured cells
11. Reevaluation of the evidence that an antibody to the insulin receptor is insulinmimetic without activating the protein tyrosine kinase activity of the receptor
12. Defined transvercion mutations at a specific position in DNA using synthetic oligodeoxy-ribonucleotides as mutagens
13. Separate domains of the insulin receptor contain sites of autophosphorylation and tyrosine kinase activity
14. Insulin rapidly stimulates phosphorylation of a 46-kDa membrane protein on tyrosine residues as well as phosphorylation of several soluble proteins in intact fat cells
15. An antipeptide antibody that specifically inhibits insulin receptor autophosphorylation and protein kinase activity
16. Synthesis, purification and characterization of the cytoplasmic domain of the human insulin receptor using a baculovirus expression system
17. Viral oncogenes and tyrosine phosphorylation
18. r 185,000 protein in intact cells
19. Requirement for receptor-intrinic tyrosine kinase activities during ligand-induced membrane ruffling of KB cells
20. Tyrosine phosphorylation of common and specific sets of cellular proteins rapidly induced by insulin, insulin-like growth factor I, and epidermal growth factor in an intact cell
21. Direct evidence that oncogenic tyrosine kinases and cyclic AMP-dependent protein kinase have homologous ATP-binding sites
22. Common elements in growth factor stimulation and oncogenic transformation 85 kd phosphoprotein and phosphatidylinositol kinase activity
23. Phosphorylation of the insulin receptor in cultured hepatoma cells and a solubilized system
24. Kinetic properties of the insulin receptor tyrosine protein kinase: activation through an insulin-stimulated tyrosine-specific, intramolecular autophosphorylation
25. Analysis of insulin receptors on human lymphoblastoid cell lines by flow cytometry
26. Human c-ros-1 gene homologous to the v-ros sequence of UR2 sarcoma virus encodes a transmembrane receptorlike molecule
27. A mutant insulin receptor with defective tyrosine kinase displays no biological activity and does not undergo endocytosis
28. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter
29. Purification of the catalytically active phosphorylated form of insulin receptor kinase by affinity chromatography with O-phosphotyrosyl-binding antibodies
30. Insulin stimulates phosphorylation of a 120-kDa glycoprotein substrate (pp120) for the receptor-associated protein kinase in intact H-35 hepatoma cells
31. After insulin binds
32. Phosphorylation activates the insulin receptor tyrosine protein kinase
33. DNA sequencing with chain-terminating inhibitors
34. The regulation of neutral amino acid transport in mammalian cells
35. Phosphorylation of synthetic insulin receptor peptides by the insulin receptor kinase and evidence that the preferred sequence containing Tyr-1150 is phosphorylated in vivo
36. Phorbol ester induced serine phosphorylation of the insulin receptor decreases its tyrosine kinase activity
37. Identification of insulin receptor tyrosine residues autophosphorylated in vitro
38. Identification of the insulin receptor tyrosine residues undergoing insulin-stimulated phosphorylation in intact rat hepatoma cells
39. Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells
40. Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes
41. Insulin-like growth factor I receptor primary structure: comparison with insulin receptor suggests structural determinants that define functional specificity
42. Simultaneous regulation of amino acid influx and efflux by system A in the hepatoma cell HTC
43. The insulin receptor and tyrosine phosphorylation
44. Kinetic properties and sites of autophosphorylation of the partially purified insulin receptor from hepatoma cells
45. r 185,000 protein in intact cells
46. Differences in the sites of phosphorylation of the insulin receptor in vivo and in vitro
47. Characterization of an endogenous substrate of the insulin receptor in cultured cells
48. A cascade of tyrosine autophosphorylation in the β-subunit activates the insulin receptor
49. Structure of the receptor for platelet-derived growth factor helps define a family of closely related growth factor receptors
50. Tyrosine phosphorylation of the insulin receptor β-subunit activates the receptor-associated tyrosine kinase activity