Mutant vasopressin precursor in the endoplasmic reticulum of the Brattleboro rat. Ultrastructural evidence from individual "vasopressin" cells localized with the light microscope by use of a new gold/silver method for immunostain enhancement

Cell and Tissue Research

Volumn 253, Issue 3, 1988, Pages 671-676

  Dr Guldenaar, S E F ^a   Pickering, B T ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Brattleboro rat; Endoplasmic reticulum; Gold silver intensification; Immunocytochemistry; Vasopressin precursor

Indexed keywords

ANIMAL CELL; CONTROLLED STUDY; CYTOLOGY; ENDOPLASMIC RETICULUM; ETHNIC OR RACIAL ASPECTS; HYPOTHALAMUS; IMMUNOCYTOCHEMISTRY; MALE; NONHUMAN; PROVASOPRESSIN; RAT; ULTRASTRUCTURE;

ANIMAL; ENDOPLASMIC RETICULUM; IMMUNOHISTOCHEMISTRY; MALE; MICROSCOPY, ELECTRON; RATS; RATS, BRATTLEBORO; RATS, INBRED STRAINS; SUPPORT, NON-U.S. GOV'T; VASOPRESSINS;

EID: 0023810935     PISSN: 0302766X     EISSN: 14320878     Source Type: Journal    
DOI: 10.1007/BF00219759     Document Type: Article

References (25)

1. Granule populations in oxytocin and abnormal perikarya of the supraoptic nucleus of homozygous Brattleboro rats: Effects of colchicine administration
2. High resolution light and electron microscopic localization of tubulin with the IGS (ImmunoGold Staining) method
3. Distribution of the C-terminal glycopeptide of the vasopressin prohormone in rat brain: an immunocytochemical study
4. Precursors of vasopressin and oxytocin
5. Immunocytochemical evidence for the presence of a mutant vasopressin precursor in the supraoptic nucleus of the homozygous Brattleboro rat
6. Immunogold-silver staining: new method of immunostaining with enhanced sensitivity
7. Expression of a mutant vasopressin gene: differential polyadenylation and read-through of the mRNA 3′ end in a frame-shift mutant
8. Immunocytochemical staining of supraoptic neurons from homozygous Brattleboro rats by use of antibodies against two domains of the mutated vasopressin precursor
9. Preembedding colloidal gold immunostaining of hypothalamic neurons: light and electron microscopic localization of beta-endorphin-immunoreactive perikarya
10. Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressin neurophysin II precursor
11. Application of the silvergold intensified 3,3-diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system of the rat brain
12. Differential immunolabelling for electron microscopy of diverse peptidergic neurons
13. Brattleboro rat hypothalamic neurons transcribe vasopressin gene. Evidence from in situ hybridization
14. Structure-function correlation in mammalian neurosecretion
15. Metal compound intensification of the electron density of diaminobenzidine
16. Immunoenzyme techniques at the electron microscopical level
17. Solitary magnocellular neurons in the homozygous Brattleboro rat have vasopressin and glycopeptide immunoreactivity
18. Single base deletion in the vasopressin gene is the cause of diabetes insipidus in Brattleboro rats
19. The mutant vasopressin gene from diabetes insipidus (Brattleboro) rats is transcribed but the message is not efficiently translated
20. Precursors in the biosynthesis of vasopressin and oxytocin in the rat. Characteristics of all the components in high performance liquid chromatography
21. Genetic approaches to the study of the regulation and actions of vasopressin
22. Gamma-aminobutyrate, gastrin releasing peptide, serotonin, somatostatin and vasopressin: ultrastructural immunocytochemical localization in presynaptic axons in the suprachiasmatic nucleus
23. Propressophysin is present in neurons at multiple sites in Wistar and homozygous Brattleboro rat brain
24. The secretory cycle of supraoptic neurons in the rat