Microwave fixation provides excellent preservation of tissue, cells and antigens for light and electron microscopy

The Histochemical Journal

Volumn 20, Issue 6-7, 1988, Pages 373-387

  Login, G R ^a   Dvorak, Ann M ^a  

^a BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; HISTOCHEMISTRY; HISTOLOGY; MICROWAVE RADIATION; NONHUMAN; TISSUE PRESERVATION; ULTRASTRUCTURE;

ANIMAL; ANTIGENS; COMPARATIVE STUDY; FEMALE; HISTOLOGICAL TECHNIQUES; HUMAN; IMMUNOENZYME TECHNIQUES; LIVER; MALE; MAST CELLS; MICROSCOPY, ELECTRON; MICROWAVES; MYOCARDIUM; SKIN; STAINING AND LABELING; SUPPORT, U.S. GOV'T, P.H.S.; TISSUE PRESERVATION;

EID: 0023796584     PISSN: 00182214     EISSN: 15736865     Source Type: Journal    
DOI: 10.1007/BF01002732     Document Type: Article

References (53)

1. The influence of protease digestion and duration of fixation on the immunostaining of keratins. A comparison of formalin and ethanol fixation
2. Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique
3. An enzyme in mast cells with properties like chymotrypsin
4. Microwave irradiation as a generator of heat for histological fixation
5. Microwave-stimulated diffusion for fast processing of tissue: reduced dehydrating, clearing, and impregnating times
6. Rapid metallic histological staining using the microwave oven
7. A fine structural study of microwave fixation of tissues
8. Rapid immunofluorescence staining of humam ranal biopsy specimens using microwave irradiation
9. The effects of different pretreatment conditions and fixation regimes on serotonin immunoreactivity: a quantitative light microscopic study
10. Vesicular uptake of eosinophil peroxidase by guinea pig basophils and by cloned mouse mast cells and granule-containing lymphoid cells
11. Immunoglobulin E-mediated degranulation of isolated human lung mast cells
12. The effect of low concentrations of glutaraldehyde on Micrococcus lysodeikticus membranes: changes in the release of membrane-associated enzymes and membrane structure
13. Isolation of rat peritoneal mast cells by centrifugation on density gradients of Percoll
14. A rapid method of staining ultrathin sections for surgical pathology TEM with the use of the microwave oven
15. Formaldehyde fixation
16. Chloracetyl esters as histochemical substrates
17. Preliminary report: microwave fixation of human tissues
18. Use of microwaves for acid- and alcohol-fast staining
19. Microwave heating patterns in simple tissue models
20. Trypsin digestion in immunoperoxidase staining
21. Microwave fixation: its potential for routine techniques, histochemistry, immunocytochemistry and electron microscopy
22. Non-ionizing electromagnetic wave effects in biological materials and systems
23. Microwave inactivation as a tool for studying the neuropharmacology of cyclic nucleotides
24. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy
25. Fundamentals of microwave-stimulated staining
26. The application of a microwave technique for the preparation of cell blocks from sputum
27. Proteolytic enzymes of mast cells
28. Microwave irradiation as a form of fixation for light and electron microscopy
29. Rapid immunoperoxidase staining of lymphocyte antigens using microwave irradiation
30. Microwave fixation versus formalin fixation of surgical and autopsy tissue
31. Microwave energy fixation for electron microscopy
32. Rapid microwave fixation of rat mast cells. I. Localization of granule chymase with an ultrastructural postembedding immunogold technique
33. Rapid microwave fixation of human tissues for light microscopic immunoperoxidase identification of diagnostically useful antigens
34. Ultrafast microwave energy fixation for electron microscopy
35. Fast and ultrafast microwave energy fixation techniques for immunofluorescence, and light and electron microscopy
36. Histological fixation by microwave heating
37. A generalized model for the interaction of microwave radiation with bound water in biological material
38. Analysis of reported physiologic effects of microwave radiation
39. Influence of fixatives on the immunoreactivity of paraffin sections
40. Microwave fixation of brain tissue as a neurochemical technique—a review
41. Microwave fixation of cells in tissue culture
42. Optimal immunoreactivity of keratin proteins in formalin-fixed, paraffin-embedded tissue requires preliminary trypsinization. An immunoperoxidase study of various tumours using polyclonal and monoclonal antibodies
43. Cyclic AMP and cyclic GMP in the rat gastric mucosa: the significance of post-mortem changes and tissue fixation on the estimation of the in vivo level
44. Activation of rat serosal mast cells by chymase, an endogenous secretory granule protease
45. Cyclic adenosine monophosphate in brain areas: microwave irradiation as a means of tissue fixation
46. Meningionmas. Diagnostic value of immunoperoxidase staining for epithelial membrane antigen
47. Effect of mast cell chymase of rat skin on intercellular matrix: a histochemical study
48. Study of brain neurochemistry utilizing rapid inactivation of brain enzyme activity by heating with microwave irradiation
49. Microwave fixation of whole peripheral nerve for rapid and efficient enzyme-linked immunosorbent assay (EL-ISA) screening of monoclonal antibodies
50. Effects of extremely low concentrations of glutaraldehyde together with shortening of fixation times on the maintenance of various enzyme activities
51. Angiotensin I conversion by human rat chymotryptic proteinases
52. Preparative purification of the rat mast cell chymase
53. Fast fixation for surgical pathology specimens