Comparison of conjugation procedures for the preparation of monoclonal antibody-enzyme conjugates

Journal of Immunological Methods

Volumn 111, Issue 2, 1988, Pages 261-270

  Jeanson, Antoinette ^a   Cloes, Jean Michel ^a   Bouchet, Mireille ^a   Rentier, Bernard ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

Author keywords

Conjugation procedure; Enzyme immunoassay; Immunochemical method; Monoclonal antibody; Peroxidase; Phosphatase

Indexed keywords

ALKALINE PHOSPHATASE; GLUTARALDEHYDE; HORSERADISH PEROXIDASE; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY;

ANIMAL CELL; CELL CULTURE; CHROMATOGRAPHY; CONJUGATION; DIAGNOSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOCYTOCHEMISTRY; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL;

ALKALINE PHOSPHATASE; ANIMAL; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; CONCANAVALIN A; DINITROFLUOROBENZENE; HYDRAZINES; IMMUNOCHEMISTRY; IMMUNOENZYME TECHNIQUES; ISOELECTRIC POINT; MICE; SUPPORT, NON-U.S. GOV'T;

EID: 0023780206     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(88)90135-4     Document Type: Article

References (14)

1. A comparative study of horseradish peroxidase conjugates prepared with a one-step and a two-step method
2. Use of fluorodinitrobenzene to identify monoclonal antibodies which are suitable for conjugation to periodate-oxidized horseradish peroxidase
3. Protein thiolation and reversible protein-protein conjugation
4. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.
5. 2b immunoglobulins from mouse serum using protein A-Sepharose
6. Preparation of enzyme-linked cytotoxic agents
7. Identification of cell surface glycoproteins by periodate-alkaline phosphatase hydrazide
8. Purification of enzyme-labeled conjugates by affinity chromatography
9. A technique for preparing defined conjugates of horseradish peroxidase and immunoglobulin
10. Specific conjugation reactions of the oligosaccharide moieties of immunoglobulins
11. Methods for the preparation of enzyme-antibody conjugates for use in enzyme immunoassay
12. A better cell line for making hybridomas secreting specific antibodies
13. Microplate enzyme immunoassays for the immunodiagnosis of virus infections
14. Comparison of three conjugation procedures for the formation of tracers for use in enzyme immunoassays