Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli

Gene

Volumn 69, Issue 2, 1988, Pages 301-315

  Amann, Egon ^a   Ochs, Birgit ^a   Abel, Karl Josef ^a  

^a Behringwerke AG   (Germany)

Author keywords

anticoagulant protein PP4; cat gene; eukaryotic protein overproduction; human coagulation factor XIIIa; lac operator; lacI coded repressor; plasmids; Recombinant DNA; trc promoter

Indexed keywords

BLOOD CLOTTING FACTOR 13A; HYBRID PROTEIN; RADIOISOTOPE; RECOMBINANT DNA;

ESCHERICHIA COLI; GENETIC ENGINEERING; GENETIC REGULATION; HEREDITY; HUMAN; HUMAN CELL; METHODOLOGY; NONHUMAN; NORMAL HUMAN; NORMAL VALUE; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROMOTER REGION;

BACTERIAL PROTEINS; BASE SEQUENCE; CODON; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENES, BACTERIAL; GENES, STRUCTURAL; GENETIC VECTORS; GENOTYPE; MOLECULAR SEQUENCE DATA; MUTATION; PLASMIDS; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; RESTRICTION MAPPING;

EID: 0023777735     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(88)90440-4     Document Type: Article

References (53)

1. ‘ATG vectors’ for regulated high-level expression of cloned genes in Escherichia coli
2. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli
3. Synthesis of human factor XIIIa in bacterial cells
4. Solid-phase synthesis of oligodeoxy-ribonucleotides by the phosphite-triester method
5. Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors
6. Plasmid vector pBR322 and its special-purpose derivatives — a review
7. Deoxynucleoside phosphoramidites — a new class of key intermediates for deoxypolynucleotide synthesis
8. A rapid alkaline extraction procedure for screening recombinant plasmid DNA
9. Isolation and characterization of two membrane-associated placental tissue proteins
10. Mechanism of action of the lexA gene product
11. Expression vectors employing λ-, trp-, lac- and lpp-derived promoters
12. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli
13. Spacing of the −10 and −35 regions in the tac promoter
14. DNA sequence for a low-level promoter of the lac repressor gene and an ‘up’ promoter mutation
15. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu
16. Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements
17. A ‘phaseshift’ fusion system for the regulation of foreign gene expression by lambda repressor in Gram-negative bacteria
18. The tac promoter a functional hybrid derived from the trp and lac promoters
19. A comprehensive set of sequence analysis programs for the VAX
20. q gene
21. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector
22. Characterization of cDNA coding for human factor XIIIa
23. Characterization of cDNA coding for human thromboplastin-inhibitor (PP4): homology with the phospholipase A2 inhibitor family
24. Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit β-globin
25. Amino acid sequence of the a subunit of human factor XIII
26. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs
27. An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs
28. The gapped duplex DNA approach to oligonucleotide-directed mutation construction
29. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
30. Sequencing end-labeled DNA with base-specific chemical cleavage
31. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in the M13 replicative form in vitro
32. Expression of Escherichia coli promoters in Brevibacterium lactofermentum using the shuttle vector pEB77003
33. Characterization in vitro of the effect of spacer length on the activity of Escherichia coli RNA polymerase at the tac promoter
34. Comparison of promoter activities of heterologous and homologous promoters in Bacillus subtilis
35. Construction and application of a novel plasmid ‘ATG vector’ for direct expression of foreign genes in Escherichia coli
36. A vector that uses phage signals for efficient synthesis of proteins in Escherichia coli
37. Inducible high level synthesis of mature human fibroblast interferon in Escherichia coli
38. Maximizing gene expression on a plasmid using recombination in vitro
39. The use of pKC30 and its derivatives for controlled expression of genes
40. Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the −35 to −10 spacing
41. DNA sequencing with chain-terminating inhibitors
42. Polymer support oligonucleotide synthesis, XVIII. Use of β-cyanoethyl -N,N-dialkylamino-N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product
43. Multicopy expression vectors carrying the lac repressor gene for regulated high-level expression of genes in Escherichia coli
44. Complete nucleotide sequence of the Escherichia coli plasmid pBR322
45. Primary structure of the blood coagulation factor XIIIa (fibrinoligase, transglutaminase) from human placenta
46. Trans-complementable copy-number mutants of plasmid ColE1
47. Herpes simplex virus type 1 glycoprotein D gene: nucleotide sequence and expression in Escherichia coli
48. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors
49. Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis