Sensitive Radiometric Assay for Chloramphenicol Acetyltransferase Using Automated HPLC

DNA

Volumn 7, Issue 7, 1988, Pages 509-513

  Zajac, Jeffrey D ^a   Gerardi, Jacqueline M ^a   Kearns, Ann K ^a   Bringhurst, F Richard ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORAMPHENICOL ACETYLTRANSFERASE; RADIOISOTOPE;

ANIMAL CELL; CELL CULTURE; HEREDITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METHODOLOGY; NONHUMAN; RAT;

ANIMAL; AUTOANALYSIS; CELL LINE; CHLORAMPHENICOL O-ACETYLTRANSFERASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TRANSFECTION;

EID: 0023724450     PISSN: 01980238     EISSN: 15577430     Source Type: Journal    
DOI: 10.1089/dna.1.1988.7.509     Document Type: Article

References (11)

1. FORREST, S.M., NG, K.W., FINDLAY, D.M., MICHELANG-ELI, V.P., LIVESEY, S.A., PARTRIDGE, M.C., ZAJAC, J.D., and MARTIN, T.J. (1985). Characterization of an osteo-blast-like clonal cell line which responds to both parathyroid hormone and calcitonin. Calcif. Tiss. Int. 37, 51–56.
2. GORMAN, C.M., MOFFAT, L.F., and HOWARD, B.H. (1982). Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2, 1044–1051.
3. GORMAN, C.M. (1985). High efficiency gene transfer into mammalian cells. In DNA Cloning, Vol. II, D.M. Glover, Ed. (IRL Press, Oxford) pp. 143–190.
4. KUEKENS, H.J., BEEK, W.M.J., and AERTS, M.M.L. (1986). High-performance liquid Chromatographic screening and confirmation methods for chloramphenicol residues in meat with off-line cartridge sample clean-up and on-line diode array UV-VIS detection. J. Chromatogr. 352, 445–453.
5. NEUMANN, J.R., MORENCY, C.A., and RUSSIAN, K.O. (1987). A novel rapid assay for chloramphenicol acetyltransferase gene expression. Biotechniques 5, 444–447.
6. NORDEEN, S.K., GREEN, P.P. III, and FOWLKES, D.M. (1987). A rapid, sensitive, and inexpensive assay for chloramphenicol acetyltransferase. DNA 6, 173–178.
7. SAKAGUCHI, K., SANTORA, A., ZIMERLING, M., CURCIO, G., AURBACH, G.D., and BRANDI, M.L. (1987). Functional epithelial cell line cloned from rat parathyroid glands. Proc. Natl. Acad. Sci. USA 84, 3269–3273.
8. SEED, B., and SHEEN, J.-Y. (1988). A simple phase extraction assay for chloramphenicol acetyltransferase activity. Gene (in press).
9. SHAW, W.V. (1975). Chloramphenicol acetyl transferase from chloramphenicol-resistant bacteria. Methods Enzymol. 43, 737–755.
10. YOUNG, S.L., JACKSON, A.E., PUETT, D., and MELNER, M.H. (1985). Detection of chloramphenicol acetyl transferase activity in transfected cells: A rapid and sensitive HPLC-based method. DNA 4, 469–475.
11. ZAJAC, J.D., OKAZAKI, T., GOODART, S.A., BRANDI, M.L., SAKAGUCHI, K., AURBACH, G., and KRONEN-BERG, H.M. (1987). Transfection and expression of the human parathyroid hormone gene in cultured bovine and rat parathyroid cells. J. Bone Min. Res. Suppl. Abstract 245.