A highly reliable, sensitive, flow cytometric/fluorometric assay for the evaluation of the anti-HIV activity of antiviral compounds in MT-4 cells

Journal of Immunological Methods

Volumn 114, Issue 1-2, 1988, Pages 27-32

  Schols, Dominique ^a   Pauwels, Rudi ^a   Vanlangendonck, Francis ^a   Balzarini, Jan ^a   De Clercq, Erik ^a  

^a REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

Author keywords

Cell viability; Flow cytometry; Fluorescein diacetate; Fluorometric assay; Human immunodeficiency virus; T4 lymphocyte

Indexed keywords

CD4 ANTIGEN;

CELL CULTURE; FLOW CYTOMETRY; FLUOROMETRY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; METHODOLOGY; PRIORITY JOURNAL; T LYMPHOCYTE;

ACQUIRED IMMUNODEFICIENCY SYNDROME; CD4-POSITIVE T-LYMPHOCYTES; CELL LINE; CELL SURVIVAL; CYTOPATHOGENIC EFFECT, VIRAL; FIXATIVES; FLOW CYTOMETRY; FLUORESCEINS; HIV; HUMAN; LEUKOCYTE COUNT; SUPPORT, NON-U.S. GOV'T; ZIDOVUDINE;

EID: 0023709365     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(88)90149-4     Document Type: Article

References (5)

1. A fluorometric assay for determining cell growth in lymphocyte proliferation and lymphokine assays
2. Inhibition of the in vitro infectivity and cytopathic effect of HTLV-III/LAV by 2′,3′-dideoxynucleosides
3. Sensitive and rapid assay on MT-4 cells for detection of antiviral compounds against the AIDS virus
4. Membrane properties of living mammalian cells studied by enzymatic hydrolysis of fluorogenic esters
5. Characterization of living normal and leukemic mouse lymphocytes by fluorescein diacetate.