A simple phase-extraction assay for chloramphenicol acyltransferase activity

Gene

Volumn 67, Issue 2, 1988, Pages 271-277

  Seed, Brian ^a   Sheen, Jenq Yunn ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

CAT; cell cultures; plant cells; reduced background; solvent extraction; transient expression; tritiated chloramphenicol

Indexed keywords

CHLORAMPHENICOL; CHLORAMPHENICOL ACETYLTRANSFERASE; RADIOISOTOPE;

CELL CULTURE; HIGHER PLANT; MAMMAL; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL;

ANIMAL; CELLS, CULTURED; CHLORAMPHENICOL O-ACETYLTRANSFERASE; COSTS AND COST ANALYSIS; METHODS; PLANTS; PROTOPLASTS; SUPPORT, NON-U.S. GOV'T;

EID: 0023687042     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(88)90403-9     Document Type: Article

References (10)

1. Secretion of Escherichia coli chloramphenicol acetyltransferase by mammalian cells
2. An immunological assay for chloramphenicol acetyltransferase
3. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells
4. A novel rapid assay for chloramphenicol acetyltransferase gene expression
5. The enzymatic acetylation of chloramphenicol by R-factor resistant Escherichia coli
6. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria
7. Chloramphenicol resistance by enzymatic acetylation comparative aspects
8. A nonchromatographic assay for expression of the chloramphenicol acetyltransferase gene in eucaryotic cells
9. Control of ColE1 plasmid replication: initial interaction of RNAI and the primer transcript is reversible
10. Affinity and hydrophobic chromatography of three variants ofchloramphenicol acetyl-transferases specified by R factors in Escherichia coli