Electroporation of Mammalian Cells with a Firefly Luciferase Expression Plasmid: Kinetics of Transient Expression Differ Markedly among Cell Types

DNA

Volumn 7, Issue 8, 1988, Pages 557-562

  Maxwell, Ian H ^a   Maxwell, Françoise ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LUCIFERASE; RECOMBINANT DNA;

ANIMAL CELL; CELL CULTURE; ELECTROPORATION; GENE EXPRESSION; GENE TRANSFER; GENETIC ENGINEERING; HEREDITY; HUMAN; HUMAN CELL; MAMMAL; METHODOLOGY; NONHUMAN; PLASMID;

CELL LINE; HUMAN; IN VITRO; LUCIFERASE; PLASMIDS; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TRANSFECTION;

EID: 0023681992     PISSN: 01980238     EISSN: 15577430     Source Type: Journal    
DOI: 10.1089/dna.1.1988.7.557     Document Type: Article

References (24)

1. ADACHI, A., GENDELMAN, H.E., KOENIG, S., FOLKS, T., WILLEY, R., RABSON, A., and MARTIN, M.A. (1986). Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. J. Virol. 59, 284–291.
2. ALWINE, J.C. (1985). Transient gene expression control: Effects of transfected DNA stability and trans-activation by viral early proteins. Mol. Cell. Biol. 5, 1034–1042.
3. BAUDHUIN, P., BEAUFAY, H., RAHMAN-LI, Y., SELLINGER, O.Z., WATTIAUX, R., JACQUES, P., and DE DUVE, C. (1964). Tissue fractionation studies. 17. Intracellular distribution of monoamine oxidase, aspartate aminotransferase, D-amino acid oxidase and catalase in rat liver. Biochem. J. 92, 179–184.
4. CHU, G., HAYAKAWA, H., and BERG, P. (1987). Electroporation for the efficient transfection of mammalian cells with DNA. Nucleic Acids Res. 15, 1311–1326.
5. DEMBIC, Z., HAAS, W., ZAMOYSKA, R., PARNES, J., STEINMETZ, M., and VON BOEHMER, H. (1987). Transfection of the CD8 gene enhances T-cell recognition. Nature 326, 510–511.
6. DE WET, J.R., WOOD, K.V., DELUCA, M., HELINSKI, D.R., and SUBRAMANI, S. (1987). Firefly luciferase gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7, 725–737.
7. EVANS, G.A., INGRAHAM, H.A., LEWIS, K., CUNNINGHAM, K., SEKI, T., MORIUCHI, T., CHANG, H.C., SILVER, J., and HYMAN, R. (1984). Expression of the thy-1 glycoprotein gene by DNA-mediated gene transfer. Proc. Natl. Acad. Sci. USA 81, 5532–5536.
8. FROMM, M., TAYLOR, L.P., and WALBOT, V. (1985). Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc. Natl. Acad. Sci. USA 82, 5824–5828.
9. FROMM, M., TAYLOR, L.P., and WALBOT, V. (1986). Stable transformation of maize after gene transfer by electroporation. Nature 319, 791–793.
10. GOLDMAN, B.M., and BLOBEL, G. (1978). Biogenesis of peroxisomes: Intracellular site of synthesis of catalase and uricase. Proc. Natl. Acad. Sci. USA 75, 5066–5070.
11. GORMAN, C.M., MOFFAT, L.F., and HOWARD, B.H. (1982). Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2, 1044–1051.
12. KELLER, G.-A., GOULD, S., DELUCA, M., and SUBRAMANI, S. (1987). Firefly luciferase is targeted to peroxisomes in mammalian cells. Proc. Natl. Acad. Sci. USA 84, 3264–3268.
13. KNUTSON, J.C., and YEE, D. (1987). Electroporation: Parameters affecting transfer of DNA into mammalian cells. Analyt. Biochem. 164, 44–52.
14. MAXWELL, I.H., MAXWELL, F., and GLODE, L.M. (1986). Regulated expression of a diphtheria toxin A-chain gene transfected into human cells: Possible strategy for inducing cancer cell suicide. Cancer Res. 46, 4660–4664.
15. NEUMANN, E., SCHAEFER-RIDDER, M., WANG, Y., and HOFSCHNEIDER, P.H. (1982). Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J. 7, 841–845.
16. NORDEEN, S.K., GREEN, P.P., III, and FOWLKES, D.M. (1987). A rapid, sensitive, and inexpensive assay for chloramphenicol acetyltransferase. DNA 6, 173–178.
17. NOVAK, T.J., and ROTHENBERG, E.V. (1986). Differential transient and long-term expression of DNA sequences introduced into T-lymphocyte lines. DNA 5, 439–451.
18. OU-LEE, T.-M., TURGEON, R., and WU, R. (1986). Expression of a foreign gene linked to either a plant-virus or a Drosophila promoter, after electroporation of protoplasts of rice, wheat, and sorghum. Proc. Natl. Acad. Sci. USA 83, 6815–6819.
19. POTTER, H., WEIR, L., and LEDER, P. (1984). Enhancer-dependent expression of human k-immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc. Natl. Acad. Sci. USA 81, 7161–7165.
20. RIGGS, C.D., and BATES, G.W. (1986). Stable transformation of tobacco by electroporation: Evidence for plasmid concatenation. Proc. Natl. Acad. Sci. USA 83, 5602–5606.
21. SIEBENLIST, U., DURAND, D.B., BRESSLER, P., HOL-BROOK, N.J., NORRIS, C.A., KAMOUN, M., KANT, J.A., and CRABTREE, G.R. (1986). Promoter region of interleukin-2 gene undergoes chromatin structure changes and confers inducibility on chloramphenicol acetyltransferase gene during activation of T cells. Mol. Cell. Biol. 6, 3042–3049.
22. SUGDEN, B., MARSH, K., and YATES, J. (1985). A vector that replicates as a plasmid can be efficiently selected in B-lympho-blasts transformed by Epstein-Barr virus. Mol. Cell. Biol. 5, 410–413.
23. TONEGUZZO, F., HAYDAY, A., and KEATING, A. (1986). Electric field-mediated DNA transfer: Transient and stable gene expression in human and mouse lymphoid cells. Mol. Cell. Biol. 6, 703–706.
24. TUR-KASPA, R., TEICHER, L., LEVINE, B.J., SKOULTCHI, A.I., and SHAFRITZ, D.A. (1986). Use of electroporation to introduce biologically active foreign genes into primary rat hepatocytes. Mol. Cell. Biol. 6, 716–718.