Dexamethasone increases adenylyl cyclase activity and expression of the α-subunit of gs in gh3 cells

Endocrinology

Volumn 121, Issue 5, 1987, Pages 1711-1715

  Chang, Fu Hsiung ^a   Bourne, Henry R ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENYLATE CYCLASE; DEXAMETHASONE; GLUCOCORTICOID; RNA;

ALPHA CHAIN; ANIMAL CELL; ANIMAL EXPERIMENT; CELL CULTURE; GENETIC ENGINEERING; HYPOPHYSIS; METHODOLOGY; NONHUMAN; RAT;

ADENYLATE CYCLASE; ANIMAL; CELL LINE; CELL MEMBRANE; DEXAMETHASONE; KINETICS; MACROMOLECULAR SYSTEMS; PITUITARY GLAND; RATS; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TRANSCRIPTION, GENETIC;

EID: 0023571767     PISSN: 00137227     EISSN: 19457170     Source Type: Journal    
DOI: 10.1210/endo-121-5-1711     Document Type: Article

References (23)

1. Davies AO, Lefkowitz RJ 1984 Regulation of /3-adrenergic receptors by steroid hormones. Annu Rev Physiol 46:119.
2. Stryer L, Bourne HR 1986 G-Proteins: a family of signal transducers. Annu Rev Cell Biol 2:391.
3. Dobner PR, Kawasaki ES, Yu L-Y, Bancroft FC 1981 Thyroid or glucocorticoid hormone induces pre-growth-hormone mRNA and its probable nuclear precursor in rat pituitary cells. Proc Natl Acad Sci USA 78:2230.
4. Cathala G, Savouret J-F, Mendez B, West BL, Karin M, Martial JA, Baxter JD 1983 A method for isolation of intact, translationally active ribonucleic acid. DNA 2:329.
5. Thomas PS 1980 Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA77:5201.
6. Southern EM 1975 Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503.
7. Feinberg AP, Vogelstein B 1983 A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6.
8. 4Ci rat pituitary tumor cells. Proc Natl Sci USA 81:6183.
9. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ 1951 Protein measurement with the Folin phenol reagent. J Biol Chem 193:265.
10. Salomon Y, Londos C, Rodbell M 1974 A highly sensitive adenylyl cyclase assay. Anal Biochem 58:541.
11. Goldman DW, Chang FH, Gifford LA, Goetzl EJ, Bourne HR 1985 Pertussis toxin inhibition of chemotactic factor-induced calcium mobilization and function in human polymorphonuclear leukocytes. J Exp Med 162:145.
12. Laemmli UK 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680.
13. Kaslow HR, Farfel Z, Johnson GL, Bourne HR 1979 Adenylate cyclase assembled in vitro: cholera toxin substrates determine different patterns of regulation by isoproterenol and guanosine 5'-triphosphate. Mol Pharmacol 15:472.
14. Farfel Z, Brickman AS, Kaslow HR, Brothers VM, Bourne HR 1980 Defect of receptor-cyclase coupling protein in pseudohypoparathyroidism. N Engl J Med 303:237.
15. Green DA, Clark RB 1982 Direct evidence for the role of the coupling proteins in forskolin activation of adenylyl cyclase. J Cyclic Nucleotide Res 8:337.
16. Darfler FJ, Mahan LC, Koachman AM, Insel PA 1982 Stimulation by forskolin of intact S49 lymphoma cells involves the nucleotide regulatory protein of adenylate cyclase. J Biol Chem 257:11901.
17. Svoboda M, Furnelle J, Eckstein F, Christophe J 1980 Guanosine 5'-0-(2-thiodiphosphate) as a competitive inhibitor of GTP in hormone or cholera toxin-stimulated pancreatic adenylate cyclase. FEBS Lett 109:275.
18. Rodan SB, Rodan GA 1986 Dexamethasone effects on /3-adrenergic receptors and adenylate cyclase regulatory proteins G„ and Gj in ROS 17/2.8 cells. Endocrinology 118:2510.
19. Johnson GS, Jaworski CJ 1983 Glucocorticoids increase GTP-dependent adenylate cyclase activity in cultured fibroblasts. Mol Pharmacol 23:648.
20. Chen TL, Feldman D 1978 Glucocorticoid potentiation of the adenosine 3',5'-monophosphate response to parathyroid hormone in cultured rat bone cells. Endocrinology 102:589.
21. 3pituitary cells, acetylcholine and vasoactive intestinal peptide antagonistically modulate adenylate cyclase, cyclic AMP content, and prolactin secretion. Mol Pharmacol 24:189.
22. 3 cells. J Biol Chem261:2684.
23. Bilezikian JP, Loeb JN 1983 The influence of hyperthyroidism and hypothyroidism on a- and /3-adrenergic receptor systems and adrenergic responsiveness. Endocr Rev 4:378.