Purified and unpurified sodium channels from eel electroplax in planar lipid bilayers

Journal of General Physiology

Volumn 90, Issue 3, 1987, Pages 375-395

  Recio Pinto, Esperanza ^a,b   Duch, Daniel S ^a,b   Rock Levinson, S ^a,b   Urban, Bernd W ^a,b  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

^b UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BATRACHOTOXIN; TETRODOTOXIN; VERATRIDINE;

ANIMAL CELL; ELECTROPHORUS; FISH; LIPID BILAYER; NONHUMAN; PROTEIN PURIFICATION; SODIUM CHANNEL;

ANIMAL; BATRACHOTOXINS; ELECTRIC CONDUCTIVITY; ELECTRIC ORGAN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ELECTROPHORUS; IN VITRO; ION CHANNELS; LIPID BILAYERS; MEMBRANE POTENTIALS; SODIUM; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TETRODOTOXIN;

EID: 0023492436     PISSN: 00221295     EISSN: 15407748     Source Type: Journal    
DOI: 10.1085/jgp.90.3.375     Document Type: Article

References (47)

1. Agnew, W. S., S. R. Levinson, J. S. Brabson, and M. A. Raftery. 1978. Purification of the tetrodotoxin-binding component associated with the voltage-sensitive sodium channel from Electrophorus electricus electroplax membranes. Proceedings of the National Academy of Sciences. 75:2606-2610.
2. Agnew, W. S., R. L. Rosenberg, and S. A. Tomiko. 1986. Reconstitution of the sodium channels from Electrophorus electricus. In Ion Channel Reconstitution. Christopher Miller, editor. Plenum Publishing Corp., New York. 307-335.
3. Andersen, O. S. 1983. Ion movement through gramicidin A channels. Single channel measurements at very high potentials. Biophysical Journal. 41:119-133.
4. + channels in planar lipid bilayers. In Ion Channel Reconstitution. Christopher Miller, editor. Plenum Publishing Corp., New York. 385-404.
5. Andersen, O. S., and R. U. Muller. 1982. Monazomycin-induced single channels. I. Characterization of the elementary conductance events. Journal of General Physiology. 80:403-426.
6. Catterall, W. A. 1986. Molecular properties of voltage-sensitive sodium channels. Annual Review of Biochemistry. 55:953-985.
7. Corbett, A. M., W. C. Zinkand, and B. K. Krueger. 1986. Saxitoxin (STX) and calcium interactions with purified sodium channels reconstituted in planar lipid bilayer. Neurosciences Abstracts. 12:45.
8. Duch, D. S. 1986. Reconstitution and functional characterization of the voltage-dependent sodium channel from eel electroplax. Ph.D. dissertation. University of Colorado Medical School, Denver, CO. 133 pp.
9. Duch, D. S., and S. R. Levinson. 1987. Neurotoxin-modulated uptake of sodium by highly purified preparations of the electroplax tetrodotoxin-binding glycopeptide reconstituted into lipid vesicles. Journal of Membrane Biology. In press.
10. Duch, D. S., S. R. Levinson, E. Recio-Pinto, and B. W. Urban. 1986. Purified and unpurified sodium channels from eel electroplax in planar bilayers. Journal of Physiology. 381:66P. (Abstr.)
11. Duch, D. S., E. Recio-Pinto, and B. W. Urban. 1987. BTX-modified, small conductance sodium channels from eel electroplax and dog brain in planar lipid bilayers. Biophysical Journal. 51:434a. (Abstr.)
12. Dunn, O. J., and V. A. Clark. 1974. Analysis of variance and regression. In Applied Statistics. John Wiley & Sons, New York. 62-86.
13. Elmer, L. W., B. J. O’Brien, T. J. Nutter, and K. J. Angelides. 1985. Physicochemical characterization of the alpha-peptide of the sodium channel from rat brain. Biochemistry. 24:8128-8137.
14. French, R. J., J. F. Worley III, M. B. Blaustein, W. O. Romine, Jr., K. K. Tam, and B. K. Krueger. 1986. Gating of batrachotoxin-activated sodium channels in lipid bilayers. In Ion Channel Reconstitution. Christopher Miller, editor. Plenum Publishing Corp., New York. 365-382.
15. Furman, R. E., J. C. Tanaka, P. Mueller, and R. L. Barchi. 1986. Voltage-dependent activation in purified reconstituted sodium channels from rabbit T-tubular membranes. Proceedings of the National Academy of Sciences. 83:488-492.
16. Garber, S. S. 1987. Permeation pathway of potassium ions in single toxin-modified sodium channels is asymmetric. Biophysical Journal. 51:9a. (Abstr.)
17. + channels activated by veratridine and batracho-toxin. Journal of General Physiology. 89:459-480.
18. Goldin, A. L., T. Snutch, H. Lubbert, A. Dowsett, J. Marshall, V. Auld, W. Downey, L. C. Fritz, H. A. Lester, R. Dunn, W. A. Catterall, and N. Davison. 1986. Messenger RNA coding for only the a subunit of the rat brain Na channel is sufficient for expression of functional channels in Xenopus oocytes. Proceedings of the National Academy of Sciences. 83:7503-7507.
19. Green, W. N., L. B. Weiss, and O. S. Andersen. 1987a. Batrachotoxin-modified sodium channels in planar lipid bilayers. Ion permeation and block. Journal of General Physiology. 89:841-872.
20. Green, W. N., L. B. Weiss, and O. S. Andersen. 1987b. Batrachotoxin-modified sodium channels in planar lipid bilayers. Characterization of saxitoxin- and tetrodotoxin-induced channel closures. Journal of General Physiology. 89:873-903.
21. +-channels into planar lipid bilayers. EMBO Journal. 3:509-515.
22. Hartshorne, R. P., and W. A. Catterall. 1984. The sodium channel from rat brain: purification and subunit composition. Journal of Biological Chemistry. 259:1667-1674.
23. Hartshorne, R. P., B. U. Keller, J. A. Talvenheimo, W. A. Catterall, and M. Montal. 1985. Functional reconstitution of the purified brain sodium channel in planar lipid bilayers. Proceedings of the National Academy of Sciences. 82:240-244.
24. Keller, B. U., R. P. Hartshorne, J. A. Talvenheimo, W. A. Catterall, and M. Montal. 1986. Sodium channels in planar lipid bilayers. Channel gating kinetics of purified sodium channels modified by batrachotoxin. Journal of General Physiology. 88:1-23.
25. Kraner, S. D., J. C. Tanaka, and R. L. Barchi. 1985. Purification and functional reconstitution of the voltage-sensitive sodium channel from rabbit t-tubular membranes. Journal of Biological Chemistry. 260:6341-6347.
26. Krueger, B. K., J. F. Worley, and R. J. French. 1983. Single sodium channels from rat brain incorporated into planar lipid bilayer membranes. Nature. 303:172-175.
27. Levinson, S. R., D. S. Duch, B. W. Urban, and E. Recio-Pinto. 1986. The sodium channel from Electrophorus electricus. Annals of the New York Academy of Sciences. 479:162-178.
28. Messner, D. J., and W. A. Catterall. 1986. The sodium channel from rat brain. Role of the β1 and β2 subunits in saxitoxin binding. Journal of Biological Chemistry. 261:211-215.
29. Miller, J. A., W. S. Agnew, and S. R. Levinson. 1983. Principal glycopeptide of the tetrodo-toxin/saxitoxin binding protein from Electrophorus electricus: isolation and partial chemical and physical characterization. Biochemistry. 22:462-470.
30. +. Journal of General Physiology. 84:665-686.
31. Noda, M., T. Ikeda, T. Kayano, H. Suzuki, H. Takeshima, M. Kurasaki, H. Takahashi, and S. Numa. 1986a. Existence of distinct sodium channel messenger RNAs in rat brain. Nature. 320:188-192.
32. Noda, M., T. Ikeda, H. Suzuki, H. Takeshima, H. Takahashi, M. Kuno, and S. Numa. 1986b. Expression of functional sodium channels from cloned cDNA. Nature. 322:826-828.
33. Noda, M., S. Shimizu, T. Tanabe, T. Takai, T. Kayano, T. Ikeda, H. Takashi, H. Nakayama, Y. Kanaoka, N. Minamino, K. Kangawa, H. Matsuo, M. A. Raftery, T. Hirose, S. Inayama, H. Hayashida, T. Miyata, and S. Numa. 1985. Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence. Nature. 312:121-127.
34. + channel. Proceedings of the National Academy of Sciences. 80:4164-4168.
35. Recio-Pinto, E., D. S. Duch, S. R. Levinson, and B. W. Urban. 1986. BTX-modified sodium channels from eel electroplax in planar bilayers. Biophysical Journal. 49:380a. (Abstr.)
36. Rosenberg, R. L. 1985. Functional reconstitution of the voltage-regulated sodium channel purified from electroplax of Electrophorus electricus. Ph.D. dissertation. Yale University, New Haven, CT. 150 pp.
37. Rosenberg, R. L., S. A. Tomiko, and W. S. Agnew. 1984a. Reconstitution of neurotoxin-modulated ion transport by the voltage-regulated sodium channel isolated from the electroplax of Electrophorus electricus. Proceedings of the National Academy of Sciences. 81:1239-1243.
38. Rosenberg, R. L., S. A. Tomiko, and W. S. Agnew. 1984b. Single channel properties of the reconstituted voltage-regulated Na channel isolated from the eel electroplax of Electrophorus electricus. Proceedings of the National Academy of Sciences. 81:5594-5598.
39. Talvenheimo, J. A., M. M. Tamkun, and W. A. Catterall. 1982. Reconstitution of neurotoxin-stimulated sodium transport by the voltage-sensitive sodium channel purified from rat brain. Journal of Biological Chemistry. 257:11868-11871.
40. Tamkun, M. M., J. A. Talvenheimo, and W. A. Catterall. 1984. The sodium channel from rat brain. Reconstitution of neurotoxin-activated ion flux and scorpion toxin binding from purified components. Journal of Biological Chemistry. 259:1676-1688.
41. Tanaka, J. C., J. F. Eccleston, and R. L. Barchi. 1983. Cation selectivity characteristics of the reconstituted sodium channel purified from rat skeletal muscle sarcolemma. Journal of Biological Chemistry. 258:7519-7526.
42. Tanaka, J. C., R. E. Furman, and R. L. Barchi. 1986. Skeletal muscle sodium channels. In Ion Channel Reconstitution. Christopher Miller, editor. Plenum Publishing Corp., New York. 277-305.
43. Tomiko, S. A., R. L. Rosenberg, M. C. Emerick, and W. S. Agnew. 1986. Fluorescence assay for neurotoxin-modulated ion transport by the reconstituted voltage-activated sodium channel isolated from eel electric organ. Biochemistry. 25:2162-2174.
44. Urban, B. W., S. B. Hladky, and D. A. Haydon. 1980. Ion movements in gramicidin pores: an example of single-file transport. Biochimica et Biophysica Acta. 602:331-354.
45. Wallenstein, S., C. L. Zucker, and J. L. Fleiss. 1980. Some statistical methods useful in circulation research. Circulation Research. 47:1-9.
46. Weigele, J. B., and R. L. Barchi. 1982. Functional reconstitution of the purified sodium channel protein from rat sarcolemma. Proceedings of the National Academy of Sciences. 79:3651-3655.
47. Weiss, L. B., W. N. Green, and O. S. Andersen. 1984. Single-channel studies on the gating of batrachotoxin (BTX)-modified sodium channels in lipid bilayers. Biophysical Journal. 45:67a.