A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coli

Gene

Volumn 57, Issue 2-3, 1987, Pages 267-272

  Hoffman, Charles S ^a   Winston, Fred ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

plasmid transformation; Recombinant DNA; Saccharomyces cerevisiae; Schizo saccharomyces pombe; Southern blot analysis

Indexed keywords

RECOMBINANT DNA;

CELL TRANSFORMATION; ESCHERICHIA COLI; FUNGUS; GENETIC ENGINEERING; METHODOLOGY; NONHUMAN; PLASMID; SACCHAROMYCES CEREVISIAE;

DNA, FUNGAL; ESCHERICHIA COLI; GENETIC VECTORS; PLASMIDS; SACCHAROMYCES CEREVISIAE; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.; TRANSFORMATION, GENETIC;

EID: 0023481280     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(87)90131-4     Document Type: Article

References (15)

1. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments
2. A complementation analysis of the restriction and modification of DNA in E. coll
3. Isolation of yeast DNA
4. Replicating plasmids in Schizosaccharomyces pombe: improvement of symmetric segregation by a new genetic element
5. A rapid, efficient method for isolating DNA from yeast
6. Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae
7. Plasmids carrying the yeast OMP decarboxylase structural and regulatory genes: transcription regulation in a foreign environment
8. Vector systems for the expression, analysis and cloning of DNA sequences in S. cerevisiae
9. DNA rearrangements associated with a transposable element in yeast
10. Detection of specific sequences among DNA fragments separated by gel electrophoresis
11. High frequency transformation of yeast: autonomous replication of hybrid DNA molecules
12. Eviction and transplacement of mutant genes in yeast
13. Analysis of the yeast SPT3 gene and identification of its product, a positive regulator of Ty transcription