cDNA cloning of alkaline phosphatase from rat osteosarcoma (ROS 17/2.8) cells

Journal of Bone and Mineral Research

Volumn 2, Issue 2, 1987, Pages 161-164

  Noda, M ^a   Yoon, K ^a   Thiede, M ^a   Buenaga, R ^a   Weiss, M ^b   Henthorn, P ^b   Harris, H ^b   Rodan, G A ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; DNA;

ANIMAL; ARTICLE; CELL LINE; ENZYMOLOGY; GENETICS; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OSTEOSARCOMA; RAT;

ALKALINE PHOSPHATASE; ANIMAL; BASE SEQUENCE; CELL LINE; CLONING, MOLECULAR; DNA; MOLECULAR SEQUENCE DATA; OSTEOSARCOMA; RATS;

EID: 0023321331     PISSN: 08840431     EISSN: 15234681     Source Type: Journal    
DOI: 10.1002/jbmr.5650020212     Document Type: Article

References (20)

1. Expression of the osteoblastic phenotype
2. Evidence that three structural genes code for human alkaline phosphatases.
3. Isolation and characterization of a cDNA encoding a human liver/bone/kidney type alkaline phosphatase.
4. Products of two common alleles at the locus for human placental alkaline phosphatase differ by seven amino acids.
5. Cloning, sequencing and chromosomal localization of human term placental alkaline phosphatase cDNA.
6. Molecular cloning and sequence analysis of human placental alkaline phosphatase.
7. Expression of alkaline phosphatase loci in mammalian tissues.
8. DNA sequencing with chain terminating inhibitors.
9. Sequencing end‐labeled DNA with base‐specificchemical cleavages.
10. Glucocorticoid regulation of alkaline phosphatase in the osteoblastic osteosarcoma cell line ROS 17/2.8.
11. 3 enriched in clonal osteoblast‐like rat osteogenic sarcoma cells.
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14. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
15. Human actin genes are single copy for α‐skeletal and α‐cardiac actin but multicopy for β‐ and α‐cytoskeletal genes: 3′ untranslated regions are isotype specific but are conserved in evolution.