High-performance liquid chromatographic assays for bufuralol 1′-hydroxylase, debrisoquine 4-hydroxylase, and dextromethorphan O-demethylase in microsomes and purified cytochrome P-450 isozymes of human liver

Analytical Biochemistry

Volumn 162, Issue 1, 1987, Pages 24-32

  Kronbach, Thomas ^a   Mathys, Daniel ^a   Gut, Josef ^a   Catin, Therese ^a   Meyer, Urs A ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

Author keywords

bufuralol; cytochrome P 450; debrisoquine, dextromethorphan; drug metabolism; HPLC; human liver

Indexed keywords

BUFURALOL 1' HYDROXYLASE; CYTOCHROME P450; DEBRISOQUINE 4 HYDROXYLASE; DEXTROMETHORPHAN O DEMETHYLASE; UNCLASSIFIED DRUG;

ARTICLE; DRUG ANALYSIS; DRUG DETERMINATION; DRUG IDENTIFICATION; DRUG METABOLISM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; LIVER; METHODOLOGY; MICROSOME; NORMAL HUMAN; PHARMACOKINETICS; PRIORITY JOURNAL;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROME P-450 CYP2D6; CYTOCHROME P-450 ENZYME SYSTEM; DEBRISOQUIN; DEXTROMETHORPHAN; ETHANOLAMINES; HUMANS; ISOENZYMES; KINETICS; MICROSOMES, LIVER; MIXED FUNCTION OXYGENASES; OXIDOREDUCTASES; OXIDOREDUCTASES, O-DEMETHYLATING; POLYMORPHISM, GENETIC;

EID: 0023258883     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/0003-2697(87)90006-6     Document Type: Article

References (15)

1. A family and population study of the genetic polymorphism of debrisoquine oxidation in a white British population.
2. Evidence for an enzymatic defect in the 4-hydroxylation of debrisoquine by human liver.