The use of fluorescence quenching in flow cytofluorometry to measure the attachment and ingestion phases in phagocytosis in peripheral blood without prior cell separation

Journal of Immunological Methods

Volumn 101, Issue 1, 1987, Pages 119-125

  Hed, J ^a   Hallden, G ^a   Johansson, S G O ^a   Larsson, P ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Flow cytofluorometry; Fluorescence quenching; Peripheral blood; Phagocytosis; Trypan blue; Yeast C3b

Indexed keywords

BLOOD; BLOOD AND HEMOPOIETIC SYSTEM; FLOW CYTOMETRY; FLUORESCENCE QUENCHING; GRANULOCYTE; HUMAN; HUMAN CELL; PHAGOCYTOSIS; PRIORITY JOURNAL;

CELL MEMBRANE; CELL SEPARATION; COMPARATIVE STUDY; COMPLEMENT 3B; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCEINS; FLUORESCENCE; GRANULOCYTES; LEUKOCYTE COUNT; LEUKOCYTES; PHAGOCYTOSIS; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SUPPORT, NON-U.S. GOV'T; THIOCYANATES; TIME FACTORS;

EID: 0023236839     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(87)90224-9     Document Type: Article

References (13)

1. Human leukocyte phagocytosis of zymosan particles measured by flow cytometry
2. A simple and rapid flow cytometric method for routine assessment of baker's yeast uptake by human polymorphonuclear leukocytes
3. Quantitation of neutrophil phagocytosis using fluorescent latex beads
4. The extinction of fluorescence by crystal violet and its use to differentiate between attached and ingested micro-organisms in phagocytosis
5. Response of polymorphonuclear leukocytes to particles opsonized with IgG or C3b
6. Differences in the ingestion mechanisms of IgG and C3b particles in phagocytosis by neutrophils
7. Differences in the association between the oxidase-dependent activity and plasma membrane receptors for IgG, C3b and concanavalin A of human neutrophils
8. The role of negative charge in the complement-dependent dissociation of IgG-mediated aggregation
9. Evaluation of flow cytometry and fluorescence microscopy for the estimation of bovine mononuclear phagocytes
10. Differentiation between attached and ingested immune complexes by a fluorescence quenching cytofluorometric assay
11. Phagocytosis: Flow cytometric quantitation with fluorescent microspheres
12. A flow cytometric method for the measurement of phagocytosis by polymorphonuclear leukocytes