RNA polymerase makes important contacts upstream from base pair -49 at the Escherichia coli galactose operon P1 promoter

Gene

Volumn 53, Issue 2-3, 1987, Pages 145-152

  Busby, Stephen ^a   Spassky, Annick ^b   Chan, Bernard ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b INSTITUT PASTEUR   (France)

Author keywords

bending and wrapping; cAMP dependent transcription; deletion analysis; DNA conformation; DNase footprinting; Recombinant DNA; transversions

Indexed keywords

RECOMBINANT DNA; RNA POLYMERASE;

DNA CONFORMATION; DNA FOOTPRINTING; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION;

BASE SEQUENCE; BINDING SITES; CYCLIC AMP; DNA, BACTERIAL; DNA-DIRECTED RNA POLYMERASES; GALACTOSE; GENE EXPRESSION REGULATION; OPERON; PROMOTER REGIONS (GENETICS); RECEPTORS, CYCLIC AMP; STRUCTURE-ACTIVITY RELATIONSHIP; SUPPORT, NON-U.S. GOV'T;

EID: 0023222006     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(87)90002-3     Document Type: Article

References (30)

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4. Segment-specific mutagenesis of the regulatory region in the Escherichia coli galactose operon: isolation of mutations reducing the initiation of transcription and translation
5. Mutations in the Escherichia coli galactose operon that define two promoters and the binding site of the cyclic AMP receptor protein
6. Deletion mutagenesis of the Escherichia coli galactose operon promoter region
7. The selection and characterisation of two novel mutations in the overlapping promoters of the Escherichia coli galactose operon
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9. DNase footprinting: a simple method for the detection of protein DNA binding specificity
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11. Dependence of DNA helix flexibility on base composition
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13. Comparison of the binding sites for the Escherichia coli cAMP receptor protein at the lactose and galactose promoters
14. On the different bindings affinities at the lac gal and malT promoter regions
15. The upstream operator of the Escherichia coli galactose operon is sufficient for repression of transcription initiated at the cyclic AMP-stimulated promoter
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19. Sequence-dependent helical periodicity of DNA
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21. Kinetics of RNA polymerase-promoter complex formation: effects of nonspecific DNA-protein interactions
22. Conformational analysis of lac promoters using nuclease activity of 1,10-phenanthroline-copper ion
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26. RNA polymerase interactions with the upstream region of the E. coli tyr T promoter
27. The locus of sequence-directed and protein-induced DNA bending
28. Deletion analysis of RNA polymerase interaction sites in the Escherichia coli lactose operon regulatory region