The effect of Ca2+ on the thermal stability of trypsin in phosphate-buffered saline solution used for harvesting of human embryonic lung fibroblast cultures

Journal of Biological Standardization

Volumn 15, Issue 3, 1987, Pages 265-270

  Brunius, G ^a   Sundbom, B L ^a  

^a Swedish Work Environment Authority   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

PRIORITY JOURNAL; ARTICLE; CELL CULTURE; CELL LINE; DRUG STABILITY; DRUG STORAGE; FIBROBLAST; HEAT; HUMAN; METABOLISM;

BUFFER; CALCIUM; SODIUM CHLORIDE; TRYPSIN;

BUFFERS; CALCIUM; CELL LINE; CELLS, CULTURED; DRUG STABILITY; DRUG STORAGE; FIBROBLASTS; HEAT; HUMAN; SODIUM CHLORIDE; SUPPORT, NON-U.S. GOV'T; TRYPSIN;

EID: 0023160893     PISSN: 00921157     EISSN: None     Source Type: Journal    
DOI: 10.1016/0092-1157(87)90029-1     Document Type: Article

References (13)

1. Location of cellular adhesions to solid substrata
2. Surface morphology of trypsinized human cells in vitro
3. Adhesion of culture cells to their substratum
4. The effect of temperature during trypsin treatment on viability and multiplication potential of single normal human and chicken fibroblasts
5. Improved medium for clonal growth of human diploid fibroblasts at low concentrations of serum protein
6. Isolation and characterization of the proteolytic enzyme component from commercially available crude trypsins
7. Trypsinogens and trypsins of various species
8. Autolysis of beta-trypsin. Influence of calcium ions and heat
9. Role du calcium dans le systéme trypsine-sérumalbumine
10. An effect of calcium ions on the activity, heat stability, and structure of trypsin
11. Comparison of the effect of calcium(II) and manganese(II) ions on trypsin autolysis
12. Plaque formation and isolation of pure lines with poliomyelitis viruses
13. A modified spectrophotometric determination of chymotrypsin trypsin and thrombin