Characterization of Adenosine Receptors in the PC 12 Pheochromocytoma Cell Line Using Radioligand Binding: Evidence for A‐2 Selectivity

Journal of Neurochemistry

Volumn 48, Issue 2, 1987, Pages 498-502

  Williams, Michael ^a,b   Abreu, Mary ^a,b   Jarvis, Michael F ^a,b   Noronha‐Blob, Lalita ^a,b  

^a NOVARTIS PHARMA AG   (Switzerland)

^b Nova Pharmaceuticals   (United States)

Author keywords

5 W Ethylcarboxamido adenosine; Adenosine agonists; A 2 adenosine receptors; PC 12 pheochromocytoma cell line

Indexed keywords

2 CHLOROADENOSINE; 6 N CYCLOPENTYLADENOSINE; 8 (2 AMINO 4 CHLOROPHENYL) 1,3 DIPROPYLXANTHINE; 8 (4 SULFOPHENYL)THEOPHYLLINE; 8 PHENYLTHEOPHYLLINE; ADENOSINE 5' (N ETHYLCARBOXAMIDE); ADENOSINE RECEPTOR; CYCLOHEXYLADENOSINE; RADIOISOTOPE; RADIOLIGAND;

6 N CYCLOHEXYLADENOSINE H 3; 6 N CYCLOPENTYLADENOSINE H 3; ADENOSINE 5' (N ETHYLCARBOXAMIDE) H 3; ANIMAL CELL; CELL MEMBRANE; CELL STRAIN; CYTOLOGY; DRUG ADMINISTRATION; DRUG COMPARISON; DRUG RECEPTOR BINDING; ENDOCRINE SYSTEM; IN VITRO STUDY; NONHUMAN; PHEOCHROMOCYTOMA CELL; PRELIMINARY COMMUNICATION; PRIORITY JOURNAL; RAT;

ADENOSINE; ADENOSINE-5'-(N-ETHYLCARBOXAMIDE); ADRENAL GLAND NEOPLASMS; ANIMAL; CELL LINE; GUANYLYL IMIDODIPHOSPHATE; MAGNESIUM; MAGNESIUM CHLORIDE; PHEOCHROMOCYTOMA; RADIOLIGAND ASSAY; RECEPTORS, PURINERGIC; SUBSTRATE SPECIFICITY;

EID: 0023154076     PISSN: 00223042     EISSN: 14714159     Source Type: Journal    
DOI: 10.1111/j.1471-4159.1987.tb04120.x     Document Type: Article

References (26)

1. A rapid and sensitive method for the quanti‐tation of microgram quantities of protein utilizing the principle of protein‐dye binding
2. 3H]phenylxanthine
3. 3H]NECA to a subtype of A2 adenosine receptor in rat striatal membranes. (Abstr)
4. 3H]NECA in rat striatal membranes
5. 50) of an enzymic reaction
6. The physiological role of adenosine in the central nervous system
7. Subclasses of adenosine receptors in the central nervous system: interaction with caffeine and related methylxanthines
8. Treatment with N‐ethyl maleimide selectively reduces adenosine receptor‐mediated decreases in cyclic AMP accumulation in rat hip‐pocampal slices
9. The action of adenosine analogs on PC 12 cells
10. Nomenclature of adenosine receptors
11. 6‐Cycloalkyl‐adenosines: potent Al selective adenosine agonists
12. LIGAND: a versatile computerized approach for characterization of ligand‐binding systems
13. Pharmacological profile of adenosine A2 receptor in PC12 cells
14. Regulation of depolarization‐dependent release of neurotransmitter by adenosine: cyclic AMP‐dependent enhancement of release from PC 12 cells
15. 6‐phenylisopropyladenosine
16. Adenosine as a neuromodulator
17. 2 receptors (Abstr.)
18. Summary of a symposium discussion on purine receptor nomenclature
19. Functionalized congeners of 1,3‐dipropyl‐8‐phenylxanthine: potent antagonists for adenosine receptor that modulate membrane adenylate cyclase in pheochromocytoma cells, platelets and fat cells
20. 3H]amine congener of 1,3‐dipropyl‐8‐phenylxanthine. A new radioligand for A2 adenosine receptors of human platelets
21. Purinergic receptors in mammalian tissues
22. Receptor binding in drug discovery and development
23. Evaluation of the binding of the Al selective adenosine radioligand, cyclopentyladenosine (CPA), to rat brain tissue
24. 3H]5′‐N‐Ethylcarbox‐amide adenosine binds to both Ra and Ri adenosine receptors in rat striatum