A Rapid, Sensitive, and Inexpensive Assay for Chloramphenicol Acetyltransferase

DNA

Volumn 6, Issue 2, 1987, Pages 173-178

  Nordeen, Steven K ^a   Green, Philip P ^a   Fowlkes, Dana M ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ACETIC ACID C 14; ACETIC ACID H 3; CHLORAMPHENICOL; CHLORAMPHENICOL ACETYLTRANSFERASE; CHLORAMPHENICOL C 14; DEXAMETHASONE; RADIOISOTOPE; UNCLASSIFIED DRUG;

DRUG ANALYSIS; DRUG DETERMINATION; ENZYME ASSAY; METHODOLOGY; NONHUMAN;

ACETIC ACID; ACETIC ACIDS; ACETYLTRANSFERASES; ANIMAL; CHLORAMPHENICOL O-ACETYLTRANSFERASE; CHROMATOGRAPHY, THIN LAYER; COMPARATIVE STUDY; GENES, REGULATOR; MICE; RECOMBINATION, GENETIC; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TRITIUM;

EID: 0023127059     PISSN: 01980238     EISSN: 15577430     Source Type: Journal    
DOI: 10.1089/dna.1987.6.173     Document Type: Article

References (7)

1. DE CROMBRUGGHE, B., PASTAN, I., SHAW, W.V., and ROSNER, J.L. (1973). Stimulation by cyclic AMP and ppGpp of chloramphenicol acetyl transferase synthesis. Nature (New Biol.) 241, 237–239.
2. GORMAN, CM., MOFFAT, L.F., and HOWARD, B.H. (1982). Recombinant genomes which express chloramphenicol acetyl-transferase in mammalian cells. Mol. Cell. Biol. 2, 1044–1051.
3. LOPATA, M.A., CLEVELAND, D.W., and SOLLNER-WEBB, B. (1984). High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA trans-fection coupled with dimethyl sulfoxide or glycerol shock treatment. Nucleic Acids Res. 12, 5707–5717.
4. OTT, M.-O., SPERLING, L., HERBOMEL, P., YANIV, M., and WEISS, M.C. (1984). Tissue-specific expression is conferred by a sequence from the 5′ end of the rat albumin gene. EMBO J. 3, 2505–2510.
5. SHAW, W.V. (1975). Chloramphenicol acetyltransferase from chloramphenicol resistant bacteria. Methods Enzymol. 43, 737–755.
6. SLEIGH, M.J. (1986). A nonchromatographic assay for expression of the chloramphenicol acetyltransferase gene in eukaryotic cells. Anal. Biochem. 156, 251–256.
7. YOUNG, S.L., JACKSON, A.E., PUETT, D., and MELNER, M.H. (1985). Detection of chloramphenicol acetyl transferase activity in transfected cells: A rapid and sensitive HPLC-based method. DNA 4, 469–475.