Inhibition of restriction endonuclease nel I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis

Nucleic Acids Research

Volumn 14, Issue 24, 1986, Pages 9679-9698

  Nakamaye, Kay L ^a   Eckstein, Fritz ^a  

^a MAX PLANCK INSTITUTE OF EXPERIMENTAL MEDICINE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHOROTHIOIC ACID; RESTRICTION ENDONUCLEASE;

ENZYME INHIBITION; GENETIC ENGINEERING; MUTAGENESIS; NONHUMAN;

DNA POLYMERASE I; DNA RESTRICTION ENZYMES; DNA, SINGLE-STRANDED; MUTAGENS; MUTATION; OLIGODEOXYRIBONUCLEOTIDES; SUPPORT, U.S. GOV'T, NON-P.H.S.; THIONUCLEOTIDES;

EID: 0023009068     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/14.24.9679     Document Type: Article

References (14)

1. Taylor, J. W., Schmidt, W., Coastick, R., Okruazek, A. and Eckstein, F. (1985) Nucleic Acids Res. 13, 8749-8764.
2. Taylor, J. W., Ott, J. and Eckstein, F. (1985) Nucleic Acids Res. 13, 8765-8785.
3. Maniatis, T., Fritsch, E. P. and Sambrook, J. (1982) Molecular Cloning - A Laboratory Manual, Cold Spring Harbor Laboratory pp. 466-467.
4. LeClerc, J. E., Istock, N. L., Saran, B. R. and Allen, R. Jr. (1984) J. Mol. Biol. 180, 217-327.
5. Kunkel, T. (1985) Proc. Natl. Acad. Sci. USA 82, 488-492.
6. Zoller, M. J. and Smith, M. (1983) Methods in Enzymol. 100, 469-500.
7. Smith, M. (1985) Ann. Rev. Genet. 19, 423-462.
8. Carter, P. (1986) Biochem. J. 237, 1-7.
9. Baas, P. D., Teertstra, W. R., van Mansfeld, A. D. M., Jansz, H. S., van der Marel, G. A., Veeneman, G. H. and van Boom, J. H. (1981) J. Mol. Biol. 152, 615-639.
10. Guo, L. H. and Wu, R. (1983) Methods in Enzymol. 100, 60-96.
11. Wu, R., Ruhen, G., Siegel, B., Jay, E., Splelman, P. and Tu, C. D. (1976) Biochemistry 15, 734-750.
12. Henikoff, S. (1984) Gene 28, 351-359.
13. Dagert, M. and Ehrlich, S. D. (1979) Gene 6, 23-28.
14. Taketo, A. (1974) J. Biochem. 75, 895-904.