Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives

MGG Molecular & General Genetics

Volumn 205, Issue 2, 1986, Pages 285-290

  Lopilato, Jane ^a   Bortner, Scott ^a   Beckwith, Jon ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Copy number; Escherichia coli; pBR322; pcnB

Indexed keywords

CHROMOSOME; ESCHERICHIA COLI; GENETIC ENGINEERING; HEREDITY; MUTANT; NONHUMAN; PLASMID; PLASMID COPY NUMBER;

BETA-GALACTOSIDASE; BETA-LACTAMASES; CHROMOSOMES, BACTERIAL; ESCHERICHIA COLI; GENES, BACTERIAL; MUTATION; NITROSOGUANIDINES; PLASMIDS; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0022921901     PISSN: 00263925     EISSN: 16174623     Source Type: Journal    
DOI: 10.1007/BF00430440     Document Type: Article

References (30)

1. Exonucleases I, III, and V are required for stability of ColE1-related plasmids in Escherichia coli
2. Isolation and characterization of an endonuclease from Escherichia coli specific for ribonucleic acid hybrid structures
3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
4. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
5. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons
6. Effects of rifampicin, streptolydigin and actinomycin D on the replication of ColE1 plasmid DNA in Escherichia coli
7. Isolation and characterization of Escherichia coli chromosomal mutants affecting plasmid copy number
8. Mutations in the DNA gyrB gene that are temperature sensitive for Lambda site-specific recombination, Mu growth, and plasmid maintenance
9. RNase H-defective mutants of Escherichia coli: A possible discriminatory role of RNase H in initiation of DNA replication
10. Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12
11. Initiation of replication of plasmid ColE1 DNA by RNA polymerase, ribonuclease H and DNA polymerase I
12. Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H
13. The rnh gene is essential for growth of Escherichia coli
14. Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin
15. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1
16. Temperatue-sensitive mutants for the replication of plasmids in Escherichia coli I. Isolation and specificity of host and plasmid mutations
17. Mutations in a new gene, secB, cause defective protein localization in Escherichia coli
18. Evidence for specificity at an early step in protein export in Escherichia coli
19. D-ribose metabolism Escherichia coli K-12: Genetics, regulation and transport
20. R6K plasmid replication: Influence of chromosomal genotype in minicellproducing strains of Escherichia coli K-12
21. Duplication and amplification of toxin genes in Vibrio cholerae
22. Escherichia coli mutants suppressing replication-defective mutations of the ColE1 plasmid
23. Novel method for detection of β-lactamase by using a chromogenic cephalosporin substrate
24. Escherichia coli mutant pleiotropically defective in the export of secreted proteins
25. Gel electrophoresis of restriction fragments
26. Transcriptional organization within an Escherichia coli cell division gene cluster: Direction of transcription of the cell separation gene envA
27. Mutimerization of high copy number plasmids causes instability: ColE1 encodes a determinant essential for plasmid monomerization and stability