A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotides

Gene

Volumn 50, Issue 1-3, 1986, Pages 313-320

  Verlaan de Vries, Matty ^a   Bogaard, Marianne E ^a   van den Elst, Hans ^b   van Boom, Jaques H ^b   van der Eb, Alex J ^a   Bos, Johannes L ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

^b LEIDEN UNIVERSITY   (Netherlands)

Author keywords

activated ras; amplimers; in vitro DNA amplification; mutation probe; oligomers; point mutations; Recombinant DNA; selective hybridization; tumor DNA

Indexed keywords

CYTOLOGY; DNA PROBE; GENE AMPLIFICATION; GENETIC ENGINEERING; HEREDITY; IN VITRO STUDY; METHODOLOGY; NONHUMAN; ONCOGENE; ONCOGENE RAS; POINT MUTATION; PRIORITY JOURNAL; SCREENING; TUMOR DNA;

CELL LINE; DNA, NEOPLASM; HUMANS; NUCLEIC ACID HYBRIDIZATION; OLIGODEOXYRIBONUCLEOTIDES; ONCOGENES; PROTO-ONCOGENES;

BOS;

EID: 0022903430     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(86)90335-5     Document Type: Article

References (20)

1. Three different mutations in codon 61 of the human N-ras gene detected by synthetic oligonucleotide hybridization
2. Amino-acid substitutions at codon 13 of the n-ras oncogene in human acute myeloid leukaemia
3. A human gastric carcinoma contains a single mutated and an amplified normal allele of the Ki-ras oncogene
4. Mutations in N-ras predominate in acute myeloid leukemia
5. Mechanism of activation of an N-ras gene in the human fibrosarcoma cell line HT1080.
6. Activation of Ki-ras2 gene in human colon and lung carcinomas by two different point mutations
7. N-ras gene activation in the RD human rhabdomyosarcoma cell line
8. Transforming genes of human hematopoietic tumors: frequent detection of ras-related oncogenes whose activation appears to be independent of tumor phenotype
9. k gene in a human ovarian carcinoma
10. Ha-ras oncogenes are activated by somatic alterations in human urinary tract tumours
11. Purification of DNA from formaldehyde fixed and paraffin embedded human tissue
12. A position 12-activated H-ras oncogene in all HS578T mammary carcinosarcoma cells but not normal mammary cells of the same patient
13. Characterization of a human colon/lung carcinoma oncogene
14. Alteration of the relative stability of dA.dT and dG.dC base pairs in DNA
15. Specific synthesis of DNA in vitro via a polymerase catalysed chain reaction
16. High frequency of N-ras activation in acute myelogenous leukemia
17. Absence of oncogene amplifications and occasional activation of N-ras in lymphoblastic leukemia of childhood
18. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia
19. Four human carcinoma cell lines with novel mutations in position 12 of c-K-ras oncogene
20. Mutations activating human c-Ha-rasI protooncogene (HRASI) induced by chemical carcinogens and depurination