Synthesis and expression of a synthetic gene for the activated human c-ha-ras protein

Japanese Journal of Cancer Research GANN

Volumn 77, Issue 1, 1986, Pages 45-51

  Miura, Kazunobu ^a   Inoue, Yasuhiko ^a   Nakamori, Hisae ^a   Iwai, Shigenori ^a   Ohtsuka, Eiko ^a   Ikehara, Morio ^b   Nishimura, Susumu ^c   Noguchi, Shigeru ^c  

^a HOKKAIDO UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c NATIONAL CANCER CENTER RESEARCH INSTITUTE   (Japan)

Author keywords

c Ha ras gene; synthetic; Tryptophan promoter

Indexed keywords

DNA; TRYPTOPHAN;

GENE EXPRESSION; GENE SYNTHESIS; HEREDITY; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; ONCOGENE; ONCOGENE C H RAS; PLASMID; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; GROWTH HORMONE; HUMANS; PLASMIDS; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENES;

EID: 0022620602     PISSN: 09105050     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Land, H., Parada, L. F. and Weinberg, R. A. Cellular oncogenes and multistep carcinogenesis. Science, 222, 771-778 (1983).
2. Tabin, C. J., Bradley, S. M., Bargmann, C. I., Weinberg, R. A., Papageorge, A. G., Scolnick, E. M., Dhar, R., Lowy, D. R. and Chang, E. H. Mechanism of activation of a human oncogene. Nature, 300, 143-149 (1982).
3. Taparowsky, E., Suard, Y., Fasano, O., Shimizu, K., Goldfarb, M. and Wigler, M. Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change. Nature, 300, 762-765 (1982).
4. Capon, D. J., Chen, E. Y., Levinson, A. D., Seeburg, P. H. and Goeddel, D. V. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue. Nature, 302, 33-37 (1983).
5. Reddy, E. P. Nucleotide sequence analysis of the T24 human bladder carcinoma oncogene. Science, 220, 1061-1063 (1983).
6. Reddy, E. P., Reynolds, R. K., Santos, E. and Barbacid, M. A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene. Nature, 300, 149-152 (1983).
7. Pincus, M. R., van Renswoude, J., Harford, J. B., Chang, E. H., Carty, R. P. and Klaus-ner, R. D. Prediction of the three-dimensional structure of the transforming region of the EJ/T24 human bladder oncogene product and its normal cellular homologue. Proc. Natl. Acad. Sci. USA, 80, 5253-5257 (1983).
8. Santos, E., Reddy, E. P., Pulciani, S., Feldmann, R. J. and Barbacid, M. Spontaneous activation of a human proto-oncogene. Proc. Natl. Acad. Sci. USA, 80, 4679-4683 (1983).
9. Srivastava, S. K., Yuasa, Y., Reynolds, S. H. and Aaronson, S. A. Effects of two major activating lesions on the structure and conformation of human ras oncogene products. Proc. Natl. Acad. Sci. USA, 82, 38-42 (1985).
10. Fasano, O., Aldrich, T., Tamanoi, F., Taparowsky, E., Furth, M. and Wigler, M. Analysis of the transforming potential of the human H-ras gene by random mutagenesis. Proc. Natl. Acad. Sci. USA, 81, 4008-4012 (1984).
11. Gibbs, J. B., Sigal, I. S., Poe, M. and Scolnick, E. M. Intrinsic GTPase activity distinguishes normal and oncogenic ras p21 molecules. Proc. Natl. Acad. Sci. USA, 81, 5704-5708 (1984).
12. Manne, V., Yamazaki, S. and Kung, H.-F. Guanosine nucleotide binding by highly purified Ha-ras-encoded p21 protein produced in Escherichia coli. Proc. Natl. Acad. Sci. USA, 81, 6953-6957 (1984).
13. McGrath, J. P., Capon, D. J., Goeddel, D. V. and Levinson, A. D. Comparative biochemical properties of normal and activated human ras p21 protein. Nature, 310, 644-649 (1984).
14. Sweet, R. W., Yokoyama, S., Kamata, T., Feramisco, J. R., Rosenberg, M. and Gross, M. The product of ras is a GTPase and the T24 oncogenic mutant is deficient in this activity. Nature, 311, 273-275 (1984).
15. Ikehara, M., Ohtsuka, E., Tokunaga, T., Taniyama, Y., Iwai, S., Kitano, K., Miyamoto, S., Ohgi, T., Sakuragawa, Y., Fujiyama, K., Ikari, T., Kobayashi, M., Miyake, T., Shibahara, S., Ono, A., Ueda, T., Tanaka, T., Baba, H., Miki, T., Sakurai, A., Oishi, T., Chisaka, O. and Matsubara, K. Synthesis of a gene for human growth hormone and its expression in Escherichia coli. Proc. Natl. Acad. Sci. USA, 81, 5956-5960 (1984).
16. Beaucage, S. L. and Caruthers, M. H. Deoxynucleoside phosphoramidites — a new class of key intermediates for deoxypoly-nucleotide synthesis. Tetrahedron Lett., 22, 1859-1862 (1981).
17. Sanger, F. Determination of nucleotide sequences in DNA. Science, 214, 1305-1312 (1981).
18. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
19. Hewick, R. M., Hunkapiller, M. W., Hood, L. E. and Dreyer, W. J. A gas-liquid solid phase peptide and protein sequenator. J. Biol. Chem., 256, 7990-7997 (1981).
20. Ikemura, T. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system. J. Mol. Biol., 151, 389-409 (1981).
21. Ito, H., Ike, Y., Ikuta, S. and Itakura, K. Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support. Nucleic Acids Res., 10, 1755-1769 (1982).
22. Yuasa, Y., Srivastava, S. K., Dunn, C. Y., Rhim, J. S., Reddy, E. P. and Aaronson, S. A. Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogene. Nature, 303, 775-779 (1983).
23. Sekiya, T., Fushimi, M., Hori, H., Hirohashi, S., Nishimura, S. and Sugimura, T. Molecular cloning and the total nucleotide sequence of the human c-Ha-ras-1 gene activated in a melanoma from a Japanese patient. Proc. Natl. Acad. Sci. USA, 81, 4771-4775 (1984).