Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes

Cell

Volumn 44, Issue 2, 1986, Pages 283-292

  Kozak, Marilyn ^a  

^a UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INITIATION FACTOR; PREPROINSULIN;

CYTOLOGY; DNA SEQUENCE; ETIOLOGY; GENETIC ENGINEERING; HEREDITY; IN VITRO STUDY; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; PLASMID; PRIORITY JOURNAL; RIBOSOME; RNA TRANSLATION;

ANIMAL; BASE SEQUENCE; BINDING SITES; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; EUKARYOTIC CELLS; HYDROGEN BONDING; MUTATION; NUCLEIC ACID CONFORMATION; PEPTIDE CHAIN INITIATION; PROINSULIN; PROTEIN PRECURSORS; RIBOSOMES; RNA, MESSENGER; RNA, RIBOSOMAL; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0022552131     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/0092-8674(86)90762-2     Document Type: Article

References (46)

1. Expression from an internal AUG codon of herpes simplex thymidine kinase gene inserted in a retrovirus vector
2. Measles virus P gene codes for two proteins
3. The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets
4. Sequence analysis of the viral core protein and the membrane-associated proteins of flavivirus West Nile Virus
5. The nucleotide sequence of a rat 18S ribosomal RNA gene and a proposal for the secondary structure of 18S rRNA
6. Two initiation sites for foot and mouth disease virus polyprotein in vivo
7. Nucleotide sequence analyses and predicted coding of bunyavirus genome RNA species
8. Initiation of translation of the cauliflower mosaic virus genome from a polycistronic mRNA evidence from deletion mutagenesis
9. Cross-linking of the anticodon of E. coli and B. subtilis acetylvalyl-tRNA to the ribosomal P site
10. Reovirus hemagglutinin mRNA codes for two polypeptides in overlapping reading frames
11. Sendai virus contains overlapping genes expressed from a single mRNA
12. SV40-transformed simian cells support the replication of early SV40 mutants
13. Large surface proteins of hepatitis B virus containing the pre-S sequence
14. Mutation of a termination codon affects src initiation
15. Binding of ribosomes to linear and circular forms of the 5′-terminal leader fragment of tobacco mosaic virus RNA
16. Inability of circular mRNA to attach to eukaryotic ribosomes
17. Evaluation of the “scanning model” for initiation of protein synthesis in eucaryotes
18. Role of ATP in binding and migration of 40S ribosomal subunits
19. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes
20. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles
21. Translation of insulin-related polypeptides from mRNAs with tandemly reiterated copies of the ribosome binding site
22. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs
23. Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulin in vivo
24. Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin
25. Influences of mRNA secondary structure on initiation by eucaryotic ribosomes
26. Sequences and properties of two ribosome binding sites from the small size class of reovirus mRNA
27. Migration of 40S ribosomal subunits on mRNA in the presence of edeine
28. Efficient expression of cloned complementary DNAs for secretory proteins after injection into Xenopus oocytes
29. Nucleotide sequence of cloned cDNA coding for preproricin
30. Nucleotide sequence of the gag gene and gag-pol junction of feline leukemia virus
31. Initiation of translation at internal AUG codons in mammalian cells
32. Eukaryotic ribosomes can recognize preproinsulin initiation codons jrrespective of their position relative to the 5′-end of mRNA
33. Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5′ of an ATG initiation codon
34. Sequencing end-labeled DNA with base-specific chemical cleavages
35. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter
36. α-Thalassaemia associated with the deletion of two nucleotides at position −2 and −3 preceding the AUG codon
37. Proximity of mRNA 5′-region and 18S rRNA in eukaryotic initiation complexes
38. A frameshift mutation in the pre-S region of the human hepatitis B virus genome allows production of surface antigen particles but eliminates binding to polymerized albumin
39. Nucleotide sequence of the genes for the simian virus 40 proteins VP2 and VP3
40. A possible novel interaction between the 3′-end of 18S rRNA and the 5′-leader sequence of many eukaryotic mRNAs
41. Identification of a new polypeptide coded by reovirus gene s1
42. Capping of eucaryotic mRNAs
43. Cryptic initiation sites in eukaryotic virus mRNAs
44. Production of human beta interferon in insect cells infected with a baculovirus expression vector
45. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor
46. The 3′ terminus of 16S rRNA: secondary structure and interaction with ribosomal protein S1