Properties of a purified pore-forming protein (perforin 1) isolated from H-2-restricted cytotoxic T cell granules

Journal of Experimental Medicine

Volumn 164, Issue 1, 1986, Pages 144-155

  Young, John Ding E ^a   Podack, Eckhard R ^b   Cohn, Zanvil A ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b NEW YORK MEDICAL COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; CALCIUM CHANNEL; CYTOLYSIS; CYTOTOXIC T LYMPHOCYTE; MAJOR HISTOCOMPATIBILITY COMPLEX RESTRICTION; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMAL; CALCIUM; CELL LINE; CELL MEMBRANE; CYTOPLASMIC GRANULES; CYTOTOXICITY, IMMUNOLOGIC; ELECTRIC CONDUCTIVITY; H-2 ANTIGENS; ION CHANNELS; LIPID BILAYERS; LYMPHOMA; MACROMOLECULAR SYSTEMS; MEMBRANE PROTEINS; MICE; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; T-LYMPHOCYTES, CYTOTOXIC;

EID: 0022494081     PISSN: 00221007     EISSN: 15409538     Source Type: Journal    
DOI: 10.1084/jem.164.1.144     Document Type: Article

References (36)

1. Berke, G. 1983. Cytotoxic T lymphocytes. How do they function?. ImmunoL Rev. 72:5.
2. Trinchieri, G., and B. Perussia. 1984. Human natural killer ceils: biologic and pathologic aspects. Lab. Invest. 50:489.
3. Herberman, R. B. 1982. Natural killer cells and their possible relevance to transplantation biology. Transplantation. 34:1.
4. Podack, E. R. 1985. Molecular mechanism of lymphocyte-mediated tumor lysis. Immunol. Today. 6:21.
5. Dourmashkin, R. R., P. Deteix, C. B. Simone, and P. A. Henkart. 1980. Electron microscopic demonstration of lesions on target cell membranes associated with antibody-dependent cellular cytotoxicity. Clin. Exp. Immunol. 43:554.
6. Podack, E. R., and G. Dennert. 1983. Cell-mediated cytolysis: assembly of two types of tubules with putative cytolytic function by cloned natural killer cells. Nature (Lond.). 302:442.
7. Dennert, G., and E. R. Podack. 1983. Cytolysis by H-2-specific T killer cells. Assembly of tubular complexes during the lytic reaction. J. Exp. Med. 157:1483.
8. Henkart, P. A., P. J. Millard, C. W. Reynolds, and M. P. Henkart. 1984. Cytolytic activity of purified cytoplasmic granules from cytotoxic rat large granular lymphocyte tumors. J. Exp. Med. 160:75.
9. Podack, E. R., and P. J. Konigsberg. 1984. Cytolytic T cell granules. Isolation, structural, biochemical, and functional characterization. J. Exp. Med. 160:695.
10. Millard, P.J., M. P. Henkart, C. W. Reynolds, and P. A. Henkart. 1984. Purification and properties of cytoplasmic granules from cytotoxic rat LGL tumors. J. Immunol. 132:3197.
11. Blumenthal, R., P. J. Millard, M. P. Henkart, C. W. Reynolds, and P. A. Henkart. 1984. Liposomes as targets for granule cytolysin from cytotoxic large granular lymphocyte tumors. Proc. Natl. Acad. Sci. USA. 81:5551.
12. Young, J. D.-E, C. F. Nathan, and Z. A. Cohn. 1985. Interaction of immune cells with target cell membranes: is there a common mechanism of killing?J. Cell. Biochem. 9(Suppl.): 161.
13. Young, J. D. -E., C. F. Náthan, E. R. Podack, M. A. Palladino, and Z. A. Cohn. 1986. Functional channel formation associated with cytotoxic T cell granules. Proc. Natl. Acad. Sci. USA. 83:150.
14. Masson, D., and J. Tschopp. 1985. Isolation of a lytic, pore-forming protein (perforin) from cytolytic T-lymphocytes. J. Biol. Chem. 260:9069.
15. Podack, E. R., J. D. -E. Young, and Z. A. Cohn. 1985. Isolation and biochemical and functional characterization of perforin 1 from cytolytic T cell granules. Proc. Natl. Acad. Sci. USA. 82:8629.
16. Nathan, C. F., J. A. Mercer-Smith, N. M. DeSantis, and M. A. Palladino. 1982. Role of oxygen in T cell-mediated cytolysis. J. Immunol. 129:2164.
17. Borregard, N., J. M. Heiple, E. R. Simons, and R. A. Clark. 1983. Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: translocation during activation. J. Cell Biol. 97:52.
18. Young, J. D.-E, H. Hengartner, E. R. Podack, and Z. A. Cohn. 1986. Purification and characterization of a cytolytic pore-forming protein from granules of cloned lymphocytes with natural killer activity. Cell. 44:849.
19. Young, J. D.-E, L. G. Leong, M. A. DiNome, and Z. A. Cohn. 1986. A semiautomated hemolysis microassay for membrane lytic proteins. Anal. Biochem. 154:649.
20. Young, J. D.-E, M. Blake, A. Mauro, and Z. A. Cohn. 1983. Properties of the major outer membrane protein from Neisseria gonorrhoeae incorporated into model lipid membranes. Proc. Natl. Acad. Sci. USA. 80:3831.
21. 32 Pi-adenosine triphosphate exchange. J. Biol. Chem. 246:5477.
22. Young, J. D.-E, T. M. Young, L. P. Lu, J. C. Unkeless, and Z. A. Cohn. 1982. Characterization of a membrane pore-forming protein from Entamoeba histolytica. J. Exp. Med. 156:1677.
23. Young, J. D.-E, J. C. Unkeless, T. M. Young, A. Mauro, and Z. A. Cohn. 1983. Role for mouse macrophage IgG Fc receptor as ligand -dependent ion channel. Nature (Lond.). 306:186.
24. Montal, M., and P. Mueller. 1972. Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc. Natl. Acad. Sci. USA. 69:3561.
25. Zimmerberg, J., F. S. Cohen, and A. Finkelstein. 1980. Fusion of phospholipid vesicles with planar phospholipid bilayer membranes. I. Discharge of vesicular contents across the planar membrane. J. Gen. Physiol. 75:241.
26. Young T. M., and J. D.-E Young. 1984. Protein-mediated intermembrane contact facilitates fusion of lipid vesicles with planar bilayers. Biochim. Biophys. Acta. 775:441.
27. Paterson, B., and J. Prives. 1973. Appearance of acetylcholine receptor in differentiating cultures of embryonic chick breast muscle. J. Cell Biol. 59:241.
28. Segal, M., and J. L. Barker. 1984. Rat hippocampal neurons in culture: properties of GABA-activated CI- ion conductance. J. Neurophysiol. 51:500.
29. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.). 227:680.
30. Young, G. P. H., J. D.-E Young, A. K. Deshpande, M. Goldstein, S. S. Koide, and Z. A. Cohn. 1984. A Ca2+-activated channel from Xenopus laevis oocyte membranes reconstituted into planar blayers. Proc. Natl. Acad. Sci. USA. 81:5155.
31. Young, J. D.-E, L. G. Leong, C.-C. Liu, A. Damiano, and Z. A. Cohn. 1986. Extracellular release of lymphocyte cytolytic pore-forming protein following ionophore stimulation. Proc. Natl. Acad. Sci. USA. In press.
32. Young, J. D.-E, C. G. B. Peterson, P. Venge, and Z. A. Cohn. 1986. Mechanism of membrane damage mediated by human eosinophil cationic protein. Nature (Lond.). In press.
33. Latorre R., and O. Alvarez. 1981. Voltage-dependent channels in planar bilayer membranes. Physiol. Rev. 61:77.
34. Schein, S. J., B. L. Kagan, and A. Finkelstein. 1978. Colicin K acts by forming voltage-dependent channels in phospholipid bilayer membranes. Nature (Lond.). 276:159.
35. Lynch, E. C., I. M. Rosenberg and C. Gider. 1982. An ion-channel forming protein produced by Entamoeba histolytica EMBO (Eur. Mol. Biol. Organ.). J. 1:801.
36. Young, J. D.-E, and Z. A. Cohn. 1985. Molecular mechanisms of cytotoxicity mediated by Entamoeba histolytica: characterization of a pore-forming protein (PFP). J. Cellular Biochem. 29:299.